論文 - 詳細
| RRC ID | 39559 |
|---|---|
| 著者 | Kato N, Yoshida H, Ono-Nita SK, Kato J, Goto T, Otsuka M, Lan K, Matsushima K, Shiratori Y, Omata M. |
| タイトル | Activation of intracellular signaling by hepatitis B and C viruses: C-viral core is the most potent signal inducer. |
| ジャーナル | Hepatology |
| Abstract |
To clarify the effects of hepatitis C virus (HCV) infection on hepatocytes, we analyzed and compared the induction of intracellular signals by HCV and hepatitis B virus (HBV) proteins. We examined the influence of 7 HCV (core, NS2, NS3, NS4A, NS4B, NS5A, and NS5B) and 4 HBV (precore, core, polymerase, and X) proteins on 5 well-defined intracellular signaling pathways associated with cell proliferation, differentiation, and apoptosis by use of a reporter assay. Viral protein-expression vectors were cotransfected into mammalian cells with reporter vectors having a luciferase gene driven by the following inducible cis-enhancer elements: the cyclic adenosine monophosphate response element, the serum response element (SRE), and the binding sites for nuclear factor kappaB (NF-kappaB), activator protein 1 (AP-1), and serum response factor (SRF). In addition, the activation of signals by HCV proteins was examined in a reporter plasmid having a natural interleukin-8 (IL-8) promoter upstream of a luciferase gene. Of 11 HCV and HBV proteins, HCV core had the strongest influence on intracellular signals, especially NF-kappaB-, AP-1-, and SRE-associated pathways. HCV core's activation level exceeded that of HBV X protein, a well-characterized transactivator of these signals. Moreover, HCV core activated the IL-8 promoter through NF-kappaB and AP-1. For the other proteins, HCV NS4B showed signal activation, but signals were activated at a lesser extent. The luciferase reporter assay, a recently introduced technique, helped in the elucidation of molecular events underlying the inflammatory and proliferation process in the liver induced by HCV. |
| 巻・号 | 32(2) |
| ページ | 405-12 |
| 公開日 | 2000-8-1 |
| DOI | 10.1053/jhep.2000.9198 |
| PMID | 10915750 |
| MeSH | Blotting, Western Hepacivirus / physiology* Hepatitis B virus / physiology* Humans Interleukin-8 / genetics NF-kappa B / physiology Promoter Regions, Genetic Signal Transduction* Trans-Activators / physiology Transcription Factor AP-1 / physiology Tumor Cells, Cultured Viral Core Proteins / physiology* Viral Regulatory and Accessory Proteins |
| IF | 14.679 |
| 引用数 | 172 |
| WOS 分野 | GASTROENTEROLOGY & HEPATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) Hep G2(RCB0459) |