RRC ID 39579
Author Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.
Title Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines.
Journal Biotechnol Bioeng
Abstract Chinese hamster ovary (CHO) cells have frequently been used in biotechnology for many years as a mammalian host cell platform for cloning and expressing genes of interest. A detailed physical chromosomal map of the CHO DG44 cell line was constructed by fluorescence in situ hybridization (FISH) imaging using randomly selected 303 BAC clones as hybridization probes (BAC-FISH). The two longest chromosomes were completely paired chromosomes; other chromosomes were partly deleted or rearranged. The end sequences of 624 BAC clones, including 287 mapped BAC clones, were analyzed and 1,119 informative BAC end sequences were obtained. Among 303 mapped BAC clones, 185 clones were used for BAC-FISH analysis of CHO K1 chromosomes and 94 clones for primary Chinese hamster lung cells. Based on this constructed physical map and end sequences, the chromosome rearrangements between CHO DG44, CHO K1, and primary Chinese hamster cells were investigated. Among 20 CHO chromosomes, eight were conserved without large rearrangement in CHO DG44, CHO K1, and primary Chinese hamster cells. This result suggested that these chromosomes were stable and essential in CHO cells and supposedly conserved in other CHO cell lines.
Volume 109(6)
Pages 1357-67
Published 2012-6-1
DOI 10.1002/bit.24347
PMID 22012683
MeSH Animals CHO Cells Chromosomes* Cricetinae Cricetulus / genetics* Gene Rearrangement* In Situ Hybridization, Fluorescence Physical Chromosome Mapping*
IF 4.26
Times Cited 42
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
DNA material Chinese hamster genomic BAC library clone (RDB13444)
Human and Animal Cells CHO-K1(RCB0285)