論文 - 詳細
| RRC ID | 39581 |
|---|---|
| 著者 | Masuta C, Yamana T, Tacahashi Y, Uyeda I, Sato M, Ueda S, Matsumura T. |
| タイトル | Development of clover yellow vein virus as an efficient, stable gene-expression system for legume species. |
| ジャーナル | Plant J |
| Abstract |
A highly infectious cDNA clone of clover yellow vein virus (pClYVV) was tested as a viral vector, especially for legume species. The genes for green fluorescent protein (GFP) and soybean glutamine synthetase (GS) were inserted between the genes for P1 and HC-Pro on pClYVV to create three recombinant plasmids: pClYVV-GFP, pClYVV-GFP-GS, and pClYVV-GFP:GS. In the former two constructs all the junctions between the inserted proteins contained the sequences of protease cleavage recognition sites, whereas the third construct expressed a fusion of GFP and GS. Western blot analyses showed that GFP and GS appeared to have been precisely excised from the viral polyprotein with the viral proteases (P1 and NIa). Under UV irradiation, green fluorescence was detected in infected broad bean, kidney bean, and soybean plants. The stability of the constructs in the symptomatic tissues was confirmed by RT-PCR and Western blot analyses. The plants expressing GS together with GFP became tolerant to the herbicide glufosinate, and flowered early. As the GS gene, one of the nodulin genes for nitrogen fixation, is expressed in legume species, this system will be useful for examining the function of genes important to legume plants. |
| 巻・号 | 23(4) |
| ページ | 539-46 |
| 公開日 | 2000-8-1 |
| DOI | 10.1046/j.1365-313x.2000.00795.x |
| PII | tpj795 |
| PMID | 10972880 |
| MeSH | Blotting, Western Fabaceae / genetics* Fabaceae / metabolism Fabaceae / virology Gene Expression* Gene Transfer Techniques* Genes, Plant* Genetic Vectors Glutamate-Ammonia Ligase / genetics Green Fluorescent Proteins Luminescent Proteins / genetics Plants, Medicinal* Potyvirus / genetics* Potyvirus / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| IF | 6.141 |
| 引用数 | 56 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCIYVV-Pst/CP (RDB08246) |