RRC ID 39593
著者 Yang D, Suzuki S, Hao LJ, Fujii Y, Yamauchi A, Yamamoto M, Nakamura M, Kumatori A.
タイトル Eosinophil-specific regulation of gp91(phox) gene expression by transcription factors GATA-1 and GATA-2.
ジャーナル J Biol Chem
Abstract The glycoprotein gp91(phox) is an essential component of the phagocyte NADPH oxidase and is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes. We previously suggested an eosinophil-specific mechanism of gp91(phox) gene expression. To elucidate the mechanism, we performed functional assays on deletion mutants of the gp91(phox) promoter in various types of gp91(phox)-expressing cells. A 10-base pair (bp) region from bp -105 to -96 of the promoter activated transcription of the gene in eosinophilic cells, but not in neutrophilic, monocytic, or B-lymphocytic cells. A 2-bp mutation introduced into the GATA site spanning bp -101 to -96 (-98GATA site) of the fragment abolished its activity. Gel shift assays using a GATA competitor and specific antibodies demonstrated that both GATA-1 and GATA-2 specifically bound to the -98GATA site with similar affinities. Individual transfection of GATA-1 and GATA-2 into Jurkat cells, which have neither endogenous GATA-1 nor GATA-2, activated the -105/+12 construct in a -98GATA site-dependent manner. Combined transfection of GATA-1 and GATA-2 activated the promoter less than transfection of GATA-1 alone. These results suggest that GATA-1 is an activator and that GATA-2 is a relative competitive inhibitor of GATA-1 in the expression of the gp91(phox) gene in human eosinophils.
巻・号 275(13)
ページ 9425-32
公開日 2000-3-31
DOI 10.1074/jbc.275.13.9425
PII S0021-9258(18)30055-3
PMID 10734088
MeSH Base Sequence Cell Line DNA Primers DNA-Binding Proteins / antagonists & inhibitors DNA-Binding Proteins / physiology* Eosinophils / metabolism* Erythroid-Specific DNA-Binding Factors GATA1 Transcription Factor GATA2 Transcription Factor Gene Expression Regulation / physiology* Humans Membrane Glycoproteins / genetics* Mutagenesis, Site-Directed NADPH Oxidase 2 NADPH Oxidases* Promoter Regions, Genetic Transcription Factors / antagonists & inhibitors Transcription Factors / physiology* Transcriptional Activation
IF 4.238
引用数 28
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞