論文 - 詳細
| RRC ID | 39595 |
|---|---|
| 著者 | Sato H, Ishihara S, Kawashima K, Moriyama N, Suetsugu H, Kazumori H, Okuyama T, Rumi MA, Fukuda R, Nagasue N, Kinoshita Y. |
| タイトル | Expression of peroxisome proliferator-activated receptor (PPAR)gamma in gastric cancer and inhibitory effects of PPARgamma agonists. |
| ジャーナル | Br J Cancer |
| Abstract |
Peroxisome proliferator-activated receptor (PPAR) gamma is expressed in human colon cancer, prostate cancer and breast cancer cells, and PPARgamma activation induces growth inhibition in these cells. PPARgamma expression in human gastric cancer cells, however, has not been fully investigated. We report the PPARgamma expression in human gastric cancer, and the effect of PPARgamma ligands on proliferation of gastric carcinoma cell lines. Immunohistochemistry was used to demonstrate the presence of PPARgamma protein in surgically resected specimens from well differentiated, moderately differentiated and poorly differentiated adenocarcinoma. We used reverse transcription-polymerase chain reaction and Northern and Western blot analyses to demonstrate PPARgamma expression in four human gastric cancer cell lines. PPARgamma agonists (troglitazone and 15-deoxy-Delta(12,14)-prostaglandin J2) showed dose-dependent inhibitory effects on the proliferation of the gastric cancer cells, and their effect was augmented by the simultaneous addition of 9- cis retinoic acid, a ligand of RXRalpha. Flow cytometry demonstrated G1 cell cycle arrest and a significant increase of annexin V-positive cells after treatment with troglitazone. These results suggest that induction of apoptosis together with G1 cell cycle arrest may be one of the mechanisms of the antiproliferative effect of PPARgamma activation in human gastric cancer cells. |
| 巻・号 | 83(10) |
| ページ | 1394-400 |
| 公開日 | 2000-11-1 |
| DOI | 10.1054/bjoc.2000.1457 |
| PII | S0007092000914575 |
| PMID | 11044367 |
| PMC | PMC2408786 |
| MeSH | Apoptosis / drug effects* Apoptosis / physiology Cell Cycle / drug effects Cell Division Flow Cytometry Gene Expression Regulation, Neoplastic* Humans Ligands Receptors, Cytoplasmic and Nuclear / agonists* Receptors, Cytoplasmic and Nuclear / biosynthesis* Stomach Neoplasms / chemistry Stomach Neoplasms / genetics Stomach Neoplasms / pathology* Transcription Factors / agonists* Transcription Factors / biosynthesis* Tretinoin / analogs & derivatives* Tretinoin / pharmacology Tumor Cells, Cultured |
| IF | 5.791 |
| 引用数 | 186 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN7(RCB0999) MKN28(RCB1000) MKN45(RCB1001) |