RRC ID 39595
著者 Sato H, Ishihara S, Kawashima K, Moriyama N, Suetsugu H, Kazumori H, Okuyama T, Rumi MA, Fukuda R, Nagasue N, Kinoshita Y.
タイトル Expression of peroxisome proliferator-activated receptor (PPAR)gamma in gastric cancer and inhibitory effects of PPARgamma agonists.
ジャーナル Br J Cancer
Abstract Peroxisome proliferator-activated receptor (PPAR) gamma is expressed in human colon cancer, prostate cancer and breast cancer cells, and PPARgamma activation induces growth inhibition in these cells. PPARgamma expression in human gastric cancer cells, however, has not been fully investigated. We report the PPARgamma expression in human gastric cancer, and the effect of PPARgamma ligands on proliferation of gastric carcinoma cell lines. Immunohistochemistry was used to demonstrate the presence of PPARgamma protein in surgically resected specimens from well differentiated, moderately differentiated and poorly differentiated adenocarcinoma. We used reverse transcription-polymerase chain reaction and Northern and Western blot analyses to demonstrate PPARgamma expression in four human gastric cancer cell lines. PPARgamma agonists (troglitazone and 15-deoxy-Delta(12,14)-prostaglandin J2) showed dose-dependent inhibitory effects on the proliferation of the gastric cancer cells, and their effect was augmented by the simultaneous addition of 9- cis retinoic acid, a ligand of RXRalpha. Flow cytometry demonstrated G1 cell cycle arrest and a significant increase of annexin V-positive cells after treatment with troglitazone. These results suggest that induction of apoptosis together with G1 cell cycle arrest may be one of the mechanisms of the antiproliferative effect of PPARgamma activation in human gastric cancer cells.
巻・号 83(10)
ページ 1394-400
公開日 2000-11-1
DOI 10.1054/bjoc.2000.1457
PII S0007092000914575
PMID 11044367
PMC PMC2408786
MeSH Apoptosis / drug effects* Apoptosis / physiology Cell Cycle / drug effects Cell Division Flow Cytometry Gene Expression Regulation, Neoplastic* Humans Ligands Receptors, Cytoplasmic and Nuclear / agonists* Receptors, Cytoplasmic and Nuclear / biosynthesis* Stomach Neoplasms / chemistry Stomach Neoplasms / genetics Stomach Neoplasms / pathology* Transcription Factors / agonists* Transcription Factors / biosynthesis* Tretinoin / analogs & derivatives* Tretinoin / pharmacology Tumor Cells, Cultured
IF 5.791
引用数 186
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MKN7(RCB0999) MKN28(RCB1000) MKN45(RCB1001)