RRC ID |
39596
|
著者 |
Mori M, Sakata I, Hirano T, Obana A, Nakajima S, Hikida M, Kumagai T.
|
タイトル |
Photodynamic therapy for experimental tumors using ATX-S10(Na), a hydrophilic chlorin photosensitizer, and diode laser.
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ジャーナル |
Jpn J Cancer Res
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Abstract |
ATX-S10(Na), a hydrophilic chlorin photosensitizer having an absorption maximum at 670 nm, is a candidate second-generation photosensitizer for use in photodynamic therapy (PDT) for cancer treatment. The effectiveness of PDT using ATX-S10(Na) and a diode laser for experimental tumors was evaluated in vitro and in vivo. In-vitro PDT using ATX-S10(Na) and the diode laser showed drug concentration-, laser dose- and drug exposure time-dependent cytotoxicity to various human and mouse tumor cell lines. In Meth-A sarcoma-implanted mice, optimal PDT conditions were found where tumors were completely eliminated without any toxicity. Against human tumor xenografts in nude mice, the combined use of 5 mg / kg ATX-S10(Na) and 200 J / cm(2) laser irradiation 3 h after ATX-S10(Na) administration showed excellent anti-tumor activity, and its efficacy was almost the same as that of PDT using 20 mg / kg porfimer sodium and a 100 J / cm(2) excimer dye laser 48 h after porfimer sodium injection. Microscopic observation of tumor tissues revealed that PDT using ATX-S10(Na) and the diode laser induced congestion, thrombus and degeneration of endothelial cells in tumor vessels, indicating that a vascular shutdown effect plays an important role in the anti-tumor activity of PDT using ATX-S10(Na) and the diode laser.
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巻・号 |
91(7)
|
ページ |
753-9
|
公開日 |
2000-7-1
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DOI |
10.1111/j.1349-7006.2000.tb01009.x
|
PMID |
10920284
|
PMC |
PMC5926412
|
MeSH |
Animals
Antineoplastic Agents / pharmacology*
Dihematoporphyrin Ether / pharmacology
HeLa Cells / drug effects
Humans
KB Cells / drug effects
Lasers
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Neoplasms, Experimental / drug therapy*
Neoplasms, Experimental / pathology
Photochemotherapy / methods*
Photosensitizing Agents / pharmacology*
Porphyrins / pharmacology*
Sarcoma, Experimental / drug therapy
Sarcoma, Experimental / pathology
Transplantation, Heterologous
|
引用数 |
26
|
リソース情報 |
ヒト・動物細胞 |
HeLa(RCB0007) |