RRC ID 39596
Author Mori M, Sakata I, Hirano T, Obana A, Nakajima S, Hikida M, Kumagai T.
Title Photodynamic therapy for experimental tumors using ATX-S10(Na), a hydrophilic chlorin photosensitizer, and diode laser.
Journal Jpn J Cancer Res
Abstract ATX-S10(Na), a hydrophilic chlorin photosensitizer having an absorption maximum at 670 nm, is a candidate second-generation photosensitizer for use in photodynamic therapy (PDT) for cancer treatment. The effectiveness of PDT using ATX-S10(Na) and a diode laser for experimental tumors was evaluated in vitro and in vivo. In-vitro PDT using ATX-S10(Na) and the diode laser showed drug concentration-, laser dose- and drug exposure time-dependent cytotoxicity to various human and mouse tumor cell lines. In Meth-A sarcoma-implanted mice, optimal PDT conditions were found where tumors were completely eliminated without any toxicity. Against human tumor xenografts in nude mice, the combined use of 5 mg / kg ATX-S10(Na) and 200 J / cm(2) laser irradiation 3 h after ATX-S10(Na) administration showed excellent anti-tumor activity, and its efficacy was almost the same as that of PDT using 20 mg / kg porfimer sodium and a 100 J / cm(2) excimer dye laser 48 h after porfimer sodium injection. Microscopic observation of tumor tissues revealed that PDT using ATX-S10(Na) and the diode laser induced congestion, thrombus and degeneration of endothelial cells in tumor vessels, indicating that a vascular shutdown effect plays an important role in the anti-tumor activity of PDT using ATX-S10(Na) and the diode laser.
Volume 91(7)
Pages 753-9
Published 2000-7-1
DOI 10.1111/j.1349-7006.2000.tb01009.x
PMID 10920284
PMC PMC5926412
MeSH Animals Antineoplastic Agents / pharmacology* Dihematoporphyrin Ether / pharmacology HeLa Cells / drug effects Humans KB Cells / drug effects Lasers Male Mice Mice, Inbred BALB C Mice, Nude Neoplasm Transplantation Neoplasms, Experimental / drug therapy* Neoplasms, Experimental / pathology Photochemotherapy / methods* Photosensitizing Agents / pharmacology* Porphyrins / pharmacology* Sarcoma, Experimental / drug therapy Sarcoma, Experimental / pathology Transplantation, Heterologous
Times Cited 26
Human and Animal Cells