| RRC ID |
39602
|
| 著者 |
Misawa E, Sakurai T, Yamada M, Hayasawa H, Motoyoshi K.
|
| タイトル |
Effects of macrophage colony-stimulating factor and interleukin-2 administration on NK1.1(+) cells in mice.
|
| ジャーナル |
Int J Immunopharmacol
|
| Abstract |
We studied the effects of M-CSF and IL-2 on NK1.1(+) cell activity in vivo and in vitro. Administration of M-CSF increased the number of splenic NK1.1(+) cells (vs. saline: P<0.01). Moreover, the combination of M-CSF and IL-2 (M-CSF+IL-2) produced a synergistic expansion of the number of NK1.1(+) cells compared with each single treatment (vs. saline: P<0.001). The NK1.1(+) cells were isolated from the spleen of each treated mouse (four treatment groups: saline, IL-2 alone, M-CSF alone, M-CSF+IL-2) and their functions (IL-2-induced proliferation, IFN-gamma production and cytostatic activity) were evaluated in vitro. The NK1.1(+) cells from M-CSF alone and M-CSF+IL-2 treated mice showed greater responsiveness in terms of IL-2-induced proliferation, production of IFN-gamma and cytostatic activity than the cells from saline and IL-2 alone treated mice. The NK activity in vivo was enhanced by the administration of M-CSF and IL-2, as assessed by the 'Lung clearance assay' (clearance of Yac-1 cells in lung). And the M-CSF+IL-2 treatment induced the highest NK activity of the four treatments. To show a practical effect of upregulation of NK activity in vivo by M-CSF and IL-2 administration, the effect of the four treatments on an experimental tumor metastasis model was examined. The IL-2 alone, M-CSF alone and M-CSF+IL-2 treatment reduced the metastasis of B16 melanoma. And the M-CSF+IL-2 treatment proved of greater benefit to the antimetastatic activity than each single treatment. Our results demonstrated that the administration of M-CSF increases the number of NK1.1(+) cells, which have good responsiveness to IL-2. Furthermore, the combination treatment of M-CSF and IL-2 in vivo augments the increase of NK1.1(+) cells. And these effects can contribute to the antimetastatic activity in vivo.
|
| 巻・号 |
22(11)
|
| ページ |
967-77
|
| 公開日 |
2000-11-1
|
| DOI |
10.1016/s0192-0561(00)00061-8
|
| PII |
S0192-0561(00)00061-8
|
| PMID |
11090705
|
| MeSH |
Animals
Antigens / analysis*
Antigens, Ly
Antigens, Surface
CHO Cells
Cricetinae
Cytotoxicity, Immunologic / drug effects
Humans
Interferon-gamma / biosynthesis
Interleukin-2 / pharmacology*
Killer Cells, Natural / drug effects*
Killer Cells, Natural / immunology
Lectins, C-Type
Lymphocyte Activation
Macrophage Colony-Stimulating Factor / pharmacology*
Macrophage-1 Antigen / analysis
Male
Melanoma, Experimental / secondary
Mice
Mice, Inbred C57BL
NK Cell Lectin-Like Receptor Subfamily B
Proteins / analysis*
|
| 引用数 |
21
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanom(RCB1283) |
