RRC ID |
39605
|
著者 |
Watanabe Y, Tenzen T, Nagasaka Y, Inoko H, Ikemura T.
|
タイトル |
Replication timing of the human X-inactivation center (XIC) region: correlation with chromosome bands.
|
ジャーナル |
Gene
|
Abstract |
The human genome is composed of long-range G+C% mosaic structures, which are thought to be related to chromosome bands. Replication timing during S phase is associated with chromosomal band zones; thus, band boundaries are thought to correspond to regions where replication timing switches. The proximal limit of the human X-inactivation center (XIC) has been localized cytologically to the junction zone between Xq13.1 and Xq13.2. Using PCR-based quantification of the newly replicated DNA from cell-cycle fractionated THP-1 cells, the replication timing in and around the XIC was determined at the genome sequence level. We found two regions where replication timing changes from the early to late period during S phase. One is located near a large inverted duplication proximal to the XIC, and the other is near the XIST locus. We propose that the 1Mb late-replicated zone (from the large inverted duplication to XIST) corresponds to a G-band Xq13.2. Several common characteristics were observed in the XIST region and the MHC class II-III junction which was previously defined as a band boundary. These characteristics included differential high-density clustering of Alu and LINE repeats, and the presence of polypurine/polypyrimidine tracts, MER41A, MER57 and MER58B.
|
巻・号 |
252(1-2)
|
ページ |
163-72
|
公開日 |
2000-7-11
|
DOI |
10.1016/s0378-1119(00)00208-0
|
PII |
S0378-1119(00)00208-0
|
PMID |
10903448
|
MeSH |
Animals
CHO Cells
Cell Cycle
Chromosome Banding
Cricetinae
DNA / genetics
DNA / metabolism
DNA Replication*
Dosage Compensation, Genetic*
Humans
Major Histocompatibility Complex / genetics
RNA, Long Noncoding
RNA, Untranslated*
Time Factors
Transcription Factors / genetics
Tumor Cells, Cultured
X Chromosome / genetics*
|
IF |
2.984
|
引用数 |
26
|
WOS 分野
|
GENETICS & HEREDITY
|
リソース情報 |
ヒト・動物細胞 |
CHO-K1 (SC)(RCB0403) |