RRC ID 39616
著者 Kitamura H, Kanehira K, Okita K, Morimatsu M, Saito M.
タイトル MAIL, a novel nuclear I kappa B protein that potentiates LPS-induced IL-6 production.
ジャーナル FEBS Lett
Abstract We have identified and characterized a novel member of the ankyrin-repeat family named 'molecule possessing ankyrin-repeats induced by lipopolysaccharide' (MAIL). The C-terminal portion of MAIL shared high sequence homology with the I kappa B family. Intraperitoneal injection of lipopolysaccharide (LPS) into mice rapidly (<0.5 h) induced MAIL mRNA in various tissues, particularly in the spleen, lymph node, and lung. Ectopically expressed MAIL was localized in the nucleus, and remarkably potentiated the LPS-induced mRNA expression and secretion of interleukin (IL)-6 in Swiss 3T3 cells. These findings indicate that MAIL is one of the nuclear I kappa B proteins and an activator of IL-6 production.
巻・号 485(1)
ページ 53-6
公開日 2000-11-17
DOI 10.1016/s0014-5793(00)02185-2
PII S0014-5793(00)02185-2
PMID 11086164
MeSH 3T3 Cells Adaptor Proteins, Signal Transducing Amino Acid Sequence Animals Cell Nucleus / chemistry Cloning, Molecular DNA, Complementary / genetics Drug Synergism Gene Expression Green Fluorescent Proteins Interleukin-6 / biosynthesis* Lipopolysaccharides / pharmacology* Luminescent Proteins / genetics Male Mice Mice, Inbred C57BL Molecular Sequence Data Nuclear Proteins / chemistry Nuclear Proteins / genetics Nuclear Proteins / pharmacology* Organ Specificity RNA, Messenger / analysis Recombinant Fusion Proteins Sequence Homology Transfection
IF 3.057
引用数 108
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 3T3 Swiss Albino(RCB1642)
遺伝子材料 pDEST12.2-MAILL (RDB03013) pDEST12.2-MAILS (RDB03014)