| RRC ID |
39626
|
| Author |
Akimoto K, Takaoka T, Sorimachi K.
|
| Title |
Development of a Simple Culture Method for the Tissues Contaminated with Microorganisms and Application to Establishment of a Fish Cell line.
|
| Journal |
Zoolog Sci
|
| Abstract |
Cell cultures are good in vitro model systems in place of using living animals. In the present study, we developed a simple culture method in which tissues were pretreated with a low concentration of sodium hypochlorite solution (NaClO) to prevent not only bacteria but also fungi. Scales removed from a goldfish (Carassius auratus) body were treated with 70% ethanol and then with 0.3% of sodium hypochlorite solution, and cultured in vitro in an atmosphere containing 0.5% CO2. The doubling time of the established cells (GAKS) was 24 hr. The GAKS cells contained alkaline phosphatase activity (8.3+/-1.1 nmol/min/mg protein) and secreted 0.32+/-0.07 pg/ml endothelin during a 3 day culture of a full monolayer sheet.
|
| Volume |
17(1)
|
| Pages |
61-3
|
| Published |
2000-1-1
|
| DOI |
10.2108/zsj.17.61
|
| PII |
0289-0003-17-1-61
|
| PMID |
18494573
|
| IF |
0.843
|
| Times Cited |
9
|
|
WOS Category
|
ZOOLOGY
|
| Resource |
| Human and Animal Cells |
GAKS(RCB0082) |
