論文 - 詳細
| RRC ID | 39642 |
|---|---|
| 著者 | Machida H, Ogawa K, Funaba M, Mizutani T, Tsujimoto M. |
| タイトル | mRNA expression of type I and type II receptors for activin, transforming growth factor-beta, and bone morphogenetic protein in the murine erythroleukemic cell line, F5-5.fl. |
| ジャーナル | Eur J Endocrinol |
| Abstract |
OBJECTIVE:Intracellular signaling of activin and transforming growth factor-beta (TGF-beta) is thought to be mediated by the same molecules (Smad2/3 and Smad4). Although differentiation of murine erythroleukemia F5-5.fl cells is induced by activin, it is not induced by TGF-beta, suggesting that at some point TGF-beta signaling is defective. The aim of this study was to investigate the unresponsiveness of F5-5.fl cells to TGF-beta. DESIGN:mRNA expression of ligands, receptors, and signal mediators for the TGF-beta family was examined in F5-5.fl cells using RT-PCR. RESULTS:Activin induced erythrodifferentiation of F5-5.fl cells in a dose-dependent manner. Neither TGF-beta1 nor bone morphogenetic protein (BMP)-4 affected the differentiation of F5-5.fl cells in the presence or absence of activin. Although mRNAs of TGF-betas (TGF-beta1, TGF-beta2 and TGF-beta3) were detected, those of inhibin/activin (alpha-, betaA- and betaB-subunits) and BMPs (BMP-2, BMP-4 and BMP-7) could not be detected in the cells, suggesting that neither activins nor BMPs are produced in F5-5.fl cells. The expression of both type I (ALK-4/ActRIB) and type II (ActRII) receptors for activin was detected in F5-5.fl cells. In contrast, while the expression of type I receptor for TGF-beta (ALK-5/TbetaRI) was detected, that of type II receptor (TbetaRII) was not. The mRNA of all Smads examined was detected in F5-5.fl cells. CONCLUSIONS:A defect in the type II receptor might cause unresponsiveness to TGF-beta in F5-5.fl cells. An erythrodifferentiation assay using F5-5.fl cells would be useful for measuring net activin activity because it would not be necessary to consider endogenous activins and BMPs. |
| 巻・号 | 143(5) |
| ページ | 705-10 |
| 公開日 | 2000-11-1 |
| DOI | 10.1530/eje.0.1430705 |
| PII | 1430705 |
| PMID | 11078996 |
| MeSH | Activin Receptors, Type I Activin Receptors, Type II Animals Bone Morphogenetic Proteins / biosynthesis* Cell Differentiation / drug effects Indicators and Reagents Leukemia, Erythroblastic, Acute / metabolism* Ligands Mice RNA, Messenger / biosynthesis* Receptors, Growth Factor / biosynthesis* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction / physiology Transforming Growth Factor beta / biosynthesis* Tumor Cells, Cultured |
| IF | 5.308 |
| 引用数 | 19 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| リソース情報 | |
| ヒト・動物細胞 | F5-5.fl(RCB0560) |