Reference - Detail
RRC ID | 39648 |
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Author | Zhang WY, Gotoh T, Oyadomari S, Mori M. |
Title | Coinduction of inducible nitric oxide synthase and arginine recycling enzymes in cytokine-stimulated PC12 cells and high output production of nitric oxide. |
Journal | Brain Res Mol Brain Res |
Abstract |
Nitric oxide (NO) is involved in many physiological and pathological processes in the brain. NO is synthesized from arginine by nitric oxide synthase (NOS), and the citrulline generated as a by-product can be recycled to arginine by argininosuccinate synthetase (AS) and argininosuccinate lyase (AL) via the citrulline-NO cycle. When neuronal PC12 cells differentiated with nerve growth factor were treated with interferon-gamma (IFNgamma) and tumor necrosis factor-alpha (TNFalpha), iNOS and AS mRNAs and proteins were markedly induced, with AL mRNA and protein being weakly induced. Cationic amino acid transporter-1 and -2 were not induced. IFNgamma or TNFalpha alone was ineffective. A large amount of NO (190 microM NO(2)(-) plus NO(3)(-) in culture medium in 24 h) was produced from arginine by cytokine-stimulated cells, and arginine could be replaced by citrulline. iNOS induction and NO production were attenuated by dexamethasone and dibutyryl cAMP and even more strongly so when combined. Therefore, a large amount of NO is produced in cytokine-stimulated PC12 cells following to induction of iNOS and citrulline-arginine recycling is important for NO production. |
Volume | 83(1-2) |
Pages | 1-8 |
Published | 2000-11-10 |
DOI | 10.1016/s0169-328x(00)00154-6 |
PII | S0169328X00001546 |
PMID | 11072090 |
MeSH | Animals Antineoplastic Agents / pharmacology Arginine / metabolism* Argininosuccinate Lyase / metabolism Argininosuccinate Synthase / metabolism Bucladesine / pharmacology Citrulline / metabolism Dexamethasone / pharmacology Gene Expression Regulation, Enzymologic / drug effects Glucocorticoids / pharmacology Interferon-gamma / pharmacology Lipopolysaccharides / pharmacology Nerve Growth Factor / pharmacology Neurons / cytology Neurons / drug effects Neurons / enzymology* Nitric Oxide / metabolism* Nitric Oxide Synthase / genetics* Nitric Oxide Synthase / metabolism* Nitric Oxide Synthase Type II PC12 Cells RNA, Messenger / analysis Rats Tumor Necrosis Factor-alpha / pharmacology* |
Times Cited | 27 |
Resource | |
Human and Animal Cells | PC-12(RCB0009) |