論文 - 詳細
| RRC ID | 39749 |
|---|---|
| 著者 | Parajuli A, Kwak DH, Dalponte L, Leikoski N, Galica T, Umeobika U, Trembleau L, Bent A, Sivonen K, Wahlsten M, Wang H, Rizzi E, De Bellis G, Naismith J, Jaspars M, Liu X, Houssen W, Fewer DP. |
| タイトル | A Unique Tryptophan C-Prenyltransferase from the Kawaguchipeptin Biosynthetic Pathway. |
| ジャーナル | Angew Chem Int Ed Engl |
| Abstract |
Cyanobactins are a rapidly growing family of linear and cyclic peptides produced by cyanobacteria. Kawaguchipeptins A and B, two macrocyclic undecapeptides reported earlier from Microcystis aeruginosa NIES-88, are shown to be products of the cyanobactin biosynthetic pathway. The 9 kb kawaguchipeptin (kgp) gene cluster was identified in a 5.26 Mb draft genome of Microcystis aeruginosa NIES-88. We verified that this gene cluster is responsible for the production of the kawaguchipeptins through heterologous expression of the kgp gene cluster in Escherichia coli. The KgpF prenyltransferase was overexpressed and was shown to prenylate C-3 of Trp residues in both linear and cyclic peptides in vitro. Our findings serve to further enhance the structural diversity of cyanobactins to include tryptophan-prenylated cyclic peptides. |
| 巻・号 | 55(11) |
| ページ | 3596-9 |
| 公開日 | 2016-3-7 |
| DOI | 10.1002/anie.201509920 |
| PMID | 26846478 |
| PMC | PMC5108402 |
| MeSH | Amino Acid Sequence Dimethylallyltranstransferase / chemistry Dimethylallyltranstransferase / metabolism* Escherichia coli / genetics Genome, Bacterial Microcystis / genetics Multigene Family Tryptophan / metabolism* |
| IF | 12.959 |
| 引用数 | 20 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY |
| リソース情報 | |
| 藻類 | NIES-88 |