論文 - 詳細
| RRC ID | 39755 |
|---|---|
| 著者 | Xiong Q, Tezuka Y, Kaneko T, Li H, Tran LQ, Hase K, Namba T, Kadota S. |
| タイトル | Inhibition of nitric oxide by phenylethanoids in activated macrophages. |
| ジャーナル | Eur J Pharmacol |
| Abstract |
Nitric oxide (NO) is one of the pro-inflammatory molecules. Some phenylethanoids have been previously shown to possess anti-inflammatory effects. Seven phenylethanoids from the stems of Cistanche deserticola, viz. isoacteoside, tubuloside B, acteoside, 2'-O-acetylacteoside, echinacoside, cistanoside A and tubuloside A, were tested for their effect on NO radical generation by activated murine macrophages. At the concentration of 100-200 microM, all the phenylethanoids reduced (6.3-62.3%) nitrite accumulation in lipopolysaccharide (0.1 microgram/ml)-stimulated J774.1 cells. At 200 microM, they inhibited by 32.2-72.4% nitrite accumulation induced by lipopolysaccharide (0.1 microgram/ml)/interferon-gamma (100 U/ml) in mouse peritoneal exudate macrophages. However, these compounds did not affect the expression of inducible nitric oxide (iNOS) mRNA, the iNOS protein level, or the iNOS activity in lipopolysaccharide-stimulated J774.1 cells. Instead, they showed a clear scavenging effect (6.9-43.9%) at the low concentrations of 2-10 microM of about 12 microM nitrite generated from an NO donor, 1-propanamine-3-hydroxy-2-nitroso-1-propylhydrazino (PAPA NONOate). These results indicate that the phenylethanoids have NO radical-scavenging activity, which possibly contributes to their anti-inflammatory effects. |
| 巻・号 | 400(1) |
| ページ | 137-44 |
| 公開日 | 2000-7-14 |
| DOI | 10.1016/s0014-2999(00)00354-x |
| PII | S0014-2999(00)00354-X |
| PMID | 10913595 |
| MeSH | Animals Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Cell Line Flavonoids* Free Radical Scavengers / pharmacology* Hydrogen-Ion Concentration Lipopolysaccharides / pharmacology Macrophage Activation Macrophages / metabolism* Male Mice Mice, Inbred ICR Nitric Oxide / antagonists & inhibitors* Nitric Oxide Synthase / antagonists & inhibitors Nitric Oxide Synthase / genetics Nitric Oxide Synthase Type II Phenols / pharmacology* Plants, Medicinal* Polymers / pharmacology* Polyphenols RNA, Messenger / analysis |
| IF | 3.263 |
| 引用数 | 87 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | J774.1(RCB0434) |