論文 - 詳細
| RRC ID | 39762 |
|---|---|
| 著者 | Iizuka T, Tanaka T, Suematsu M, Miura S, Watanabe T, Koike R, Ishimura Y, Ishii H, Miyasaka N, Miyasaka M. |
| タイトル | Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats. |
| ジャーナル | J Immunol |
| Abstract |
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is essential for lymphocyte trafficking to gut-associated lymphoid tissues and is implicated in inflammatory disorders in the gut and pancreatic islets. In this study, we examined the functional role of MAdCAM-1 during rat ontogeny using newly generated specific mAb. As previously observed in mice and humans, MAdCAM-1 was preferentially expressed in high endothelial venules (HEV) in gut-associated lymphoid tissues and venules of lamina propria in adult rats. Lymphocyte rolling and adhesion on HEV in Peyer's patches (PP) were completely abrogated with neutralizing anti-MAdCAM-1 mAb, in agreement with the notion that MAdCAM-1 is the principal HEV ligand for lymphocyte rolling and adhesion in adult PP. In the developing gastrointestinal tract, MAdCAM-1 was widely expressed in the venules of the lamina propria of fetal rats. In addition, MAdCAM-1 was also expressed in follicular dendritic cells in the neonatal PP. Interestingly, MAdCAM-1 expression was found also in nonmucosal tissues during ontogeny. MAdCAM-1 was transiently expressed in blood vascular endothelial cells in the fetal skin and neonatal thymus. Notably, MAdCAM-1-positive blood vessels were localized mainly in the cortico-medullary junction in the neonatal thymus and about 10-20% of thymocytes, most of which were either CD4, CD8 double positive or single positive specifically reacted with soluble MAdCAM-1 via integrin alpha4beta7. After birth, MAdCAM-1 expression in thymus blood vessels disappeared and concomitantly, the soluble MAdCAM-1-reactive thymocytes were rapidly down-regulated. Our results suggest that MAdCAM-1 functions as a vascular addressin in not only mucosal, but also nonmucosal lymphoid tissues during ontogeny. |
| 巻・号 | 164(5) |
| ページ | 2463-71 |
| 公開日 | 2000-3-1 |
| DOI | 10.4049/jimmunol.164.5.2463 |
| PII | ji_v164n5p2463 |
| PMID | 10679083 |
| MeSH | Aging / immunology Animals Animals, Newborn / immunology Antibodies, Monoclonal / biosynthesis Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / pharmacology Antibody Specificity Cell Adhesion / immunology Cell Adhesion Molecules Cell Movement / immunology Embryonic and Fetal Development / immunology* Endothelium, Lymphatic / cytology Endothelium, Lymphatic / immunology Female Humans Immunoglobulins / biosynthesis* Immunoglobulins / immunology Intestinal Mucosa / growth & development Intestinal Mucosa / immunology Male Mice Mice, Inbred BALB C Mucoproteins / biosynthesis* Mucoproteins / immunology Peyer's Patches / metabolism Rats Rats, Wistar Skin / immunology Skin / metabolism Thymus Gland / immunology Thymus Gland / metabolism |
| IF | 4.886 |
| 引用数 | 41 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CT14-G4(RCB0212) |