| RRC ID |
39762
|
| 著者 |
Iizuka T, Tanaka T, Suematsu M, Miura S, Watanabe T, Koike R, Ishimura Y, Ishii H, Miyasaka N, Miyasaka M.
|
| タイトル |
Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats.
|
| ジャーナル |
J Immunol
|
| Abstract |
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is essential for lymphocyte trafficking to gut-associated lymphoid tissues and is implicated in inflammatory disorders in the gut and pancreatic islets. In this study, we examined the functional role of MAdCAM-1 during rat ontogeny using newly generated specific mAb. As previously observed in mice and humans, MAdCAM-1 was preferentially expressed in high endothelial venules (HEV) in gut-associated lymphoid tissues and venules of lamina propria in adult rats. Lymphocyte rolling and adhesion on HEV in Peyer's patches (PP) were completely abrogated with neutralizing anti-MAdCAM-1 mAb, in agreement with the notion that MAdCAM-1 is the principal HEV ligand for lymphocyte rolling and adhesion in adult PP. In the developing gastrointestinal tract, MAdCAM-1 was widely expressed in the venules of the lamina propria of fetal rats. In addition, MAdCAM-1 was also expressed in follicular dendritic cells in the neonatal PP. Interestingly, MAdCAM-1 expression was found also in nonmucosal tissues during ontogeny. MAdCAM-1 was transiently expressed in blood vascular endothelial cells in the fetal skin and neonatal thymus. Notably, MAdCAM-1-positive blood vessels were localized mainly in the cortico-medullary junction in the neonatal thymus and about 10-20% of thymocytes, most of which were either CD4, CD8 double positive or single positive specifically reacted with soluble MAdCAM-1 via integrin alpha4beta7. After birth, MAdCAM-1 expression in thymus blood vessels disappeared and concomitantly, the soluble MAdCAM-1-reactive thymocytes were rapidly down-regulated. Our results suggest that MAdCAM-1 functions as a vascular addressin in not only mucosal, but also nonmucosal lymphoid tissues during ontogeny.
|
| 巻・号 |
164(5)
|
| ページ |
2463-71
|
| 公開日 |
2000-3-1
|
| DOI |
10.4049/jimmunol.164.5.2463
|
| PII |
ji_v164n5p2463
|
| PMID |
10679083
|
| MeSH |
Aging / immunology
Animals
Animals, Newborn / immunology
Antibodies, Monoclonal / biosynthesis
Antibodies, Monoclonal / chemistry
Antibodies, Monoclonal / pharmacology
Antibody Specificity
Cell Adhesion / immunology
Cell Adhesion Molecules
Cell Movement / immunology
Embryonic and Fetal Development / immunology*
Endothelium, Lymphatic / cytology
Endothelium, Lymphatic / immunology
Female
Humans
Immunoglobulins / biosynthesis*
Immunoglobulins / immunology
Intestinal Mucosa / growth & development
Intestinal Mucosa / immunology
Male
Mice
Mice, Inbred BALB C
Mucoproteins / biosynthesis*
Mucoproteins / immunology
Peyer's Patches / metabolism
Rats
Rats, Wistar
Skin / immunology
Skin / metabolism
Thymus Gland / immunology
Thymus Gland / metabolism
|
| IF |
4.886
|
| 引用数 |
41
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CT14-G4(RCB0212) |
