RRC ID 39763
Author Kobayashi S, Sakae K, Suzuki Y, Shinozaki K, Okada M, Ishiko H, Kamata K, Suzuki K, Natori K, Miyamura T, Takeda N.
Title Molecular cloning, expression, and antigenicity of Seto virus belonging to genogroup I Norwalk-like viruses.
Journal J Clin Microbiol
Abstract The viral capsid protein of the Seto virus (SeV), a Japanese strain of genogroup I Norwalk-like viruses (NLVs), was expressed as virus-like particles using a baculovirus expression system. An antigen detection enzyme-linked immunosorbent assay based on hyperimmune antisera to recombinant SeV was highly specific to homologous SeV-like strains but not heterologous strains in stools, allowing us type-specific detection of NLVs.
Volume 38(9)
Pages 3492-4
Published 2000-9-1
DOI 10.1128/JCM.38.9.3492-3494.2000
PMID 10970413
PMC PMC87416
MeSH Animals Antigens, Viral / analysis Blotting, Southern Caliciviridae / classification* Caliciviridae / genetics Caliciviridae / immunology* Caliciviridae Infections / virology* Capsid / genetics* Capsid / metabolism Cloning, Molecular Enzyme-Linked Immunosorbent Assay / methods Feces / virology Gastroenteritis / virology* Humans Molecular Sequence Data Norwalk virus / classification Norwalk virus / genetics Norwalk virus / immunology Rabbits Recombinant Proteins / immunology Reverse Transcriptase Polymerase Chain Reaction
IF 5.897
Times Cited 28
WOS Category MICROBIOLOGY
Resource
Human and Animal Cells Sf9(RCB0563)