論文 - 詳細
| RRC ID | 39765 |
|---|---|
| 著者 | Pai R, Sasaki E, Tarnawski AS. |
| タイトル | Helicobacter pylori vacuolating cytotoxin (VacA) alters cytoskeleton-associated proteins and interferes with re-epithelialization of wounded gastric epithelial monolayers. |
| ジャーナル | Cell Biol Int |
| Abstract |
In previous studies, we demonstrated that Helicobacter pylori vacuolating cytotoxin (VacA) inhibits gastric epithelial cell proliferation and inhibits epidermal growth factor (EGF)-activated signal transduction. Cell proliferation and migration, both essential for mucosal healing are dependent on the cell cytoskeleton. Other investigators demonstrated that VacA induces vacuolation of eukaryotic cells. Since in some cells, control of actin cytoskeleton involves GTP-binding proteins of Rho family, in this study we examined whether VacA affects wound re-epithelialization, cell cytoskeleton-associated proteins Rho, Rac1 in a gastric epithelial (RGM1) cell monolayer wound model, and whether these changes correlate with vacuolation. VacA treatment significantly inhibited wound re-epithelialization, cell proliferation vs control. VacA-induced cell vacuolation strongly correlated with inhibition of wound re-epithelialization. Furthermore, VacA reduced Rac-1 protein expression and distribution, and C3-mediated ADP-ribosylation of Rho. These findings suggest that VacA may interfere with repair of gastric mucosal injury and ulcer re-epithelialization by altering cytoskeleton-dependent cell functions and signaling. |
| 巻・号 | 24(5) |
| ページ | 291-301 |
| 公開日 | 2000-1-1 |
| DOI | 10.1006/cbir.2000.0510 |
| PII | S1065-6995(00)90510-9 |
| PMID | 10805963 |
| MeSH | ADP Ribose Transferases / pharmacology Actins / metabolism Animals Bacterial Proteins / metabolism* Bacterial Proteins / pharmacology Bacterial Toxins / metabolism* Bacterial Toxins / pharmacology Botulinum Toxins* Cell Division / drug effects Cell Line Cell Movement / drug effects Cytoplasm / drug effects Cytoplasm / metabolism Cytoplasm / pathology Cytoskeletal Proteins / drug effects Cytoskeletal Proteins / metabolism* DNA / biosynthesis Epithelial Cells / drug effects Epithelial Cells / metabolism Epithelial Cells / pathology Gastric Mucosa / drug effects Gastric Mucosa / metabolism* Gastric Mucosa / pathology* Helicobacter pylori / enzymology Mitomycin / pharmacology Rats Stress, Physiological / metabolism Vacuoles / drug effects Wound Healing* / drug effects rac1 GTP-Binding Protein / metabolism rho GTP-Binding Proteins / metabolism |
| IF | 2.571 |
| 引用数 | 22 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |