| RRC ID |
39800
|
| Author |
Nakayama Y, Aoki Y.
|
| Title |
Mechanism responsible for the formation of focal swellings on injured neuronal processes using a novel in vitro model of axonal injury.
|
| Journal |
Forensic Sci Int
|
| Abstract |
A novel in vitro model of axonal injury using PC12 cells was designed to introduce traumatic alterations on neuronal processes and to identify mechanisms responsible for the formation of focal swellings by observation with phase-contrast and transmission electron microscopes. The injury on the processes was developed by one-dimensional, horizontal oscillation. Phase-contrast microscopic observation on the injured processes showed their terminal increase in diameter. Long term observation of cellular responses to the mechanical insult disclosed that the terminal swelling coincided with the detachment of growth cones from the culture plate. The finding suggests that the detachment of the growth cone would destroy a cytoskeletal network, which determines and maintains the cell shapes, and then cause the spherical deformation of the processes. Ultramicroscopically, the processes with terminal swellings regenerated the growth cones by the cytoskeletal reconstruction.
|
| Volume |
113(1-3)
|
| Pages |
245-9
|
| Published |
2000-9-11
|
| DOI |
10.1016/s0379-0738(00)00217-6
|
| PII |
S0379073800002176
|
| PMID |
10978633
|
| MeSH |
Animals
Biomechanical Phenomena
Cell Culture Techniques / methods
Cytoskeleton / pathology
Cytoskeleton / ultrastructure
Diffuse Axonal Injury / etiology*
Diffuse Axonal Injury / pathology*
Disease Models, Animal*
Growth Cones / pathology
Growth Cones / ultrastructure
Microscopy, Electron, Scanning Transmission
Microscopy, Phase-Contrast
PC12 Cells*
Rats
|
| IF |
2.108
|
| Times Cited |
4
|
|
WOS Category
|
MEDICINE, LEGAL
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
