論文 - 詳細
| RRC ID | 39809 |
|---|---|
| 著者 | Seki N, Yamada A, Suefuji Y, Mine T, Tanaka S, Gomi SY, Kawagoe N, Koufuji K, Itoh K. |
| タイトル | Establishment and epitope analysis of allo-specific cytotoxic T lymphocytes at a tumor site recognizing a spouse's HLA-A0206 molecule. |
| ジャーナル | Am J Reprod Immunol |
| Abstract |
PROBLEM:The molecular basis of allo-reactivity in reproductive immunity has not been fully clarified. METHOD OF STUDY:Cytotoxic T lymphocytes (CTLs) were established from tumor-infiltrating lymphocytes (TILs). The allo-reactivity of the CTLs against various tumor cell lines or human leukocyte antigen (HLA)-A allele-transfected COS-7 cells was measured by 51Cr-release or interferon-gamma production assay. RESULTS AND CONCLUSIONS:We have established CTLs reacting to an HLA-A0206 molecule that matched a spouse's HLA-A allele from the TILs of a 68-year-old multiparous patient with gastric cancer. The amino acids at positions 66 and 88 in the alpha1 domain of HLA-A0206, both of which were common in the other HLA-A2 subtypes, were involved in the recognition by the CTLs. Endogenous peptides in the groove were not involved in the recognition. These results suggest the presence of long-lasting memory CTLs raised by the reproduction process, and may facilitate a better understanding of the molecular basis of allo-recognition during reproduction. |
| 巻・号 | 43(3) |
| ページ | 167-73 |
| 公開日 | 2000-3-1 |
| DOI | 10.1111/j.8755-8920.2000.430306.x |
| PMID | 10735593 |
| MeSH | Aged Alleles Animals COS Cells Epitopes, T-Lymphocyte / analysis* Female HLA-A2 Antigen / genetics HLA-A2 Antigen / immunology* HLA-A2 Antigen / metabolism Humans Immunologic Memory Lymphocytes, Tumor-Infiltrating / immunology Male Spouses* Stomach Neoplasms / immunology* T-Lymphocytes, Cytotoxic / immunology* T-Lymphocytes, Cytotoxic / metabolism Transfection Tumor Cells, Cultured |
| IF | 2.739 |
| 引用数 | 0 |
| WOS 分野 | IMMUNOLOGY REPRODUCTIVE BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MKN28(RCB1000) |