論文 - 詳細
| RRC ID | 39811 |
|---|---|
| 著者 | Tominaga K, Higuchi K, Tsuno M, Watanabe T, Fujiwara Y, Kim S, Arakawa T, Iwao H, Kuroki T. |
| タイトル | Induction of signal transduction pathways in rat gastric epithelial cells stimulated with interleukin-1beta. |
| ジャーナル | Aliment Pharmacol Ther |
| Abstract |
BACKGROUND:Interleukin-1beta (IL-1beta) participates in cell growth, differentiation and apoptosis via activation of several kinases in a variety of cells. Mitogen-activated protein (MAP) kinases are important intermediates of the signal transduction pathway from the cell surface to the nucleus, leading to activation of transcription factors. There are no reports on the effect of IL-1beta on these pathways in gastric epithelial cells. AIM:To investigate whether IL-1beta activates MAP kinases [extracellular signal-regulated kinases (ERKs), c-Jun NH2-terminal kinases (JNKs) and p38 MAP kinase (p38 MAPK)] and nuclear factor (NF)-kappaB. a transcription factor, in gastric epithelial cells (RGM1). METHODS:The activities of ERKs and JNKs were estimated by in-gel kinase assay, and p38 MAPK activity was measured by in vitro kinase assay at various time points (0-40 min) after addition of IL-1beta (100 pg/mL) for 20 min. The activity of NF-kappaB was analysed using gel mobility shift assay at times from 0 to 4 h after addition of IL-1beta. RESULTS:Activity of ERKs was detectable at 10 min, peaked at 20 min, and continued at increased levels until 40 min. Activity of both JNKs and p38 MAPK were detectable during 5-20 min, and then decreased within 40 min. Activation of NF-kappaB occurred at 30 min, and increased activity continued for 6 h. Interleukin-1beta activated MAP kinases and NF-kappaB in RGM1 cells. CONCLUSION:The activation induced by this cytokine may play an important role in the initiation of the inflammatory process in gastric mucosa. |
| 巻・号 | 14 Suppl 1 |
| ページ | 101-8 |
| 公開日 | 2000-4-1 |
| DOI | 10.1046/j.1365-2036.2000.014s1101.x |
| PMID | 10807411 |
| MeSH | Animals Enzyme Induction Epithelial Cells / physiology* Gastric Mucosa / cytology Gastric Mucosa / enzymology* Inflammation Interleukin-1 / pharmacology* Mice Mitogen-Activated Protein Kinases / metabolism* NF-kappa B / metabolism* Rats Signal Transduction* |
| IF | 7.515 |
| 引用数 | 11 |
| WOS 分野 | PHARMACOLOGY & PHARMACY GASTROENTEROLOGY & HEPATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |