RRC ID 39815
Author Mie M, Ohgushi H, Yanagida Y, Haruyama T, Kobatake E, Aizawa M.
Title Osteogenesis coordinated in C3H10T1/2 cells by adipogenesis-dependent BMP-2 expression system.
Journal Tissue Eng
Abstract A novel tissue engineering for bone formation has been proposed, to make osteoblast differentiation balanced by transfecting the mesenchymal stem cells with a gene encoding human bone morphogenetic protein-2 (hBMP-2) under the control of adipocyte specific lipoprotein lipase (LPL) promoter. Due to the promoter specificity, the initiation of BMP transcription is dependent on adipogenesis. For 14-day culture in the presence of ascorbic acid (asc) and beta-glycerophosphate (gly), nontransfected mouse embryonic fibroblast C3H10T1/2 (10T1/2) cells showed extensive accumulation of lipid droplets and adipocyte specific enzyme glycerol-3-phosphate dehydrogenase (G3PDH) mRNA expression, but exhibited neither BMP-2 expression, high alkaline phosphatase (ALP) activity which reflects osteoblast phenotype. On the other hand, transfected 10T1/2 cells showed hBMP-2 expression, high ALP activity and low level of G3PDH. mRNA expression accompanied with minimal lipid droplets. These results indicate that 10T1/2 cells are proved to be differentiated with maintaining coordinated balance of adipogenesis and osteogenesis, when they are transfected by the gene encoding hBMP-2 under the control of LPL promoter.
Volume 6(1)
Pages 9-18
Published 2000-2
DOI 10.1089/107632700320847
PMID 10941196
MeSH Adipocytes / cytology Adipocytes / physiology* Animals Ascorbic Acid / pharmacology Base Sequence Biomedical Engineering Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins / genetics Bone Morphogenetic Proteins / physiology* Cell Differentiation Cell Line DNA Primers / genetics Gene Expression Humans Lipoprotein Lipase / genetics Mice Osteoblasts / cytology Osteoblasts / physiology Osteogenesis / physiology* Promoter Regions, Genetic / drug effects RNA, Messenger / genetics RNA, Messenger / metabolism Transfection Transforming Growth Factor beta*
IF 3.508
Times Cited 18
Human and Animal Cells