RRC ID 39853
Author Ohsawa M, Mizunuma H, Kagami I, Miyamoto S, Kanuma T, Ibuki Y.
Title Effect of 1,25(OH)2D3 on expression of estrogen receptor-alpha mRNA on rat osteosarcoma cell line (ROS 17/2.8).
Journal Life Sci.
Abstract In order to investigate the regulatory mechanisms of estrogen receptors (ER) in bone cells, changes in ER-alpha mRNA levels of rat osteosarcoma cell line (ROS 17/2.8) before and after exposure to 1,25(OH)2D3 and 17-beta estradiol respectively were measured by quantitative polymerase chain reaction using an internal standard. ER mRNA levels in the ROS 17/2.8 cultured with the medium alone had 5.029 +/- 1.623 mol/g total RNA x 10(-13) and were not statistically different from those cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-12) M and less. ER mRNA levels in the ROS 17/2.8 cell line showed a small but a significant increase as a result of stimulation by 1,25(OH)2D3 at concentrations of 10(-10) and 10(-11) M. However, ER mRNA levels in ROS 17/2.8 cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-9) M were not statistically different from those of the control. On the other hand, the expression of ER in ROS 17/2.8 cells cultured for 3 hours with various doses of 1,25(OH)2D3 showed, by immunoblotting methods, a significant increase at the dose of 10(-10) M in the expression of ER. Although a physiological significance is obscure, these observations suggest that 1,25(OH)2D3 plays a part in the expression of ER in ROS 17/2.8. No significant changes were seen in the expression of ER mRNA and the synthesis of ER as a result of stimulation by the estradiol.
Volume 66(25)
Pages 2465-72
Published 2000
DOI 10.1016/s0024-3205(00)80006-5
PII S0024320500005774
PMID 10894089
MeSH Animals Estrogen Receptor alpha Estrogens / pharmacology* Gene Expression / drug effects Osteosarcoma / metabolism* Osteosarcoma / pathology RNA, Messenger / biosynthesis Rats Receptors, Estrogen / biosynthesis* Receptors, Estrogen / drug effects Receptors, Estrogen / genetics Reverse Transcriptase Polymerase Chain Reaction Steroid Hydroxylases / pharmacology* Tumor Cells, Cultured
IF 3.234
Times Cited 4
WOS Category MEDICINE, RESEARCH & EXPERIMENTAL PHARMACOLOGY & PHARMACY
Resource
Human and Animal Cells