| RRC ID |
39853
|
| 著者 |
Ohsawa M, Mizunuma H, Kagami I, Miyamoto S, Kanuma T, Ibuki Y.
|
| タイトル |
Effect of 1,25(OH)2D3 on expression of estrogen receptor-alpha mRNA on rat osteosarcoma cell line (ROS 17/2.8).
|
| ジャーナル |
Life Sci
|
| Abstract |
In order to investigate the regulatory mechanisms of estrogen receptors (ER) in bone cells, changes in ER-alpha mRNA levels of rat osteosarcoma cell line (ROS 17/2.8) before and after exposure to 1,25(OH)2D3 and 17-beta estradiol respectively were measured by quantitative polymerase chain reaction using an internal standard. ER mRNA levels in the ROS 17/2.8 cultured with the medium alone had 5.029 +/- 1.623 mol/g total RNA x 10(-13) and were not statistically different from those cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-12) M and less. ER mRNA levels in the ROS 17/2.8 cell line showed a small but a significant increase as a result of stimulation by 1,25(OH)2D3 at concentrations of 10(-10) and 10(-11) M. However, ER mRNA levels in ROS 17/2.8 cultured in the presence of 1,25(OH)2D3 at concentrations of 10(-9) M were not statistically different from those of the control. On the other hand, the expression of ER in ROS 17/2.8 cells cultured for 3 hours with various doses of 1,25(OH)2D3 showed, by immunoblotting methods, a significant increase at the dose of 10(-10) M in the expression of ER. Although a physiological significance is obscure, these observations suggest that 1,25(OH)2D3 plays a part in the expression of ER in ROS 17/2.8. No significant changes were seen in the expression of ER mRNA and the synthesis of ER as a result of stimulation by the estradiol.
|
| 巻・号 |
66(25)
|
| ページ |
2465-72
|
| 公開日 |
2000-1-1
|
| DOI |
10.1016/s0024-3205(00)80006-5
|
| PII |
S0024320500005774
|
| PMID |
10894089
|
| MeSH |
Animals
Estrogen Receptor alpha
Estrogens / pharmacology*
Gene Expression / drug effects
Osteosarcoma / metabolism*
Osteosarcoma / pathology
RNA, Messenger / biosynthesis
Rats
Receptors, Estrogen / biosynthesis*
Receptors, Estrogen / drug effects
Receptors, Estrogen / genetics
Reverse Transcriptase Polymerase Chain Reaction
Steroid Hydroxylases / pharmacology*
Tumor Cells, Cultured
|
| IF |
3.647
|
| 引用数 |
4
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
ROS-17/2.8-5(RCB0462) |
