| RRC ID |
39854
|
| Author |
Saida K, Uchide T, Usui A, Gao XD, Tomizuka N, Oka S, Masuda H.
|
| Title |
The endothelin-2/vasoactive intestinal contractor gene: expression and promoter activity in PC12 rat pheochromocytoma cells.
|
| Journal |
J Cardiovasc Pharmacol
|
| Abstract |
In order to understand the physiological roles of vasoactive intestinal contractor (VIC)/endothelin-2 (ET-2), we examined the expression of this peptide by specific reverse transcriptase polymerase chain reaction (RT-PCR) analysis and found that PC12 rat pheochromocytoma cells express the VIC gene. The 5'-flanking 1.0 kilo base pair (kb) region of the mouse VIC gene is sufficient to express a secreted alkaline phosphatase (SEAP) reporter gene in transiently transfected PC12 cells. The 1.0 kb promoter region may contain cis-acting elements that determine the rate of the VIC gene transcription in PC12 cells.
|
| Volume |
36(5 Suppl 1)
|
| Pages |
S9-11
|
| Published |
2000-11-1
|
| DOI |
10.1097/00005344-200036051-00005
|
| PMID |
11078321
|
| MeSH |
Animals
Endothelin-2 / genetics*
Genes, Reporter
Intercellular Signaling Peptides and Proteins
PC12 Cells
Peptides / genetics*
Promoter Regions, Genetic*
RNA, Messenger / analysis
Rats
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
2.598
|
| Times Cited |
1
|
|
WOS Category
|
CARDIAC & CARDIOVASCULAR SYSTEMS
PHARMACOLOGY & PHARMACY
|
| Resource |
| Human and Animal Cells |
PC-12(RCB0009) |
