| RRC ID |
39864
|
| 著者 |
Trevanich S, Miyamoto T, Harada Y, Honjoh K, Hatano S.
|
| タイトル |
Rapid detection of enterotoxigenic Escherichia coli O6 in water by using monoclonal antibody and a photon-counting television camera.
|
| ジャーナル |
J Food Prot
|
| Abstract |
Monoclonal antibodies (MAbs) raised against Escherichia coli O6:H16 were screened against 15 strains of E. coli and 19 non-E. coli bacteria. A MAb-luminescence assay using MAb-5.8, which shows no cross-reactions with non-E. coli bacteria, and a photon-counting television camera were developed for rapid enumeration of E. coli O6:H16 in water. The membrane filter that retained bacteria was boiled for 5 min in a buffer and incubated with biotinylated MAb-5.8. After incubation with streptavidin-peroxidase conjugate, it was reacted with luminol-based reaction mixture. Luminous image and light intensity of the filter was recorded with a Biocell Counter. Levels of E. coli O6 higher than 7 x 10(3) CFU were detected by the MAb-luminescence assay when E. coli O6 was spotted onto the membrane filter. The sample that contained E. coli O6:H16 was filtered through a membrane filter, and the filter that retained bacteria was incubated on a filter paper soaked with nutrient broth supplemented with 0.5% NaCl at 37 degrees C for 6 h. The number of light emission points on the filter correlated well with initial E. coli O6:H16 counts within the range of 1 to 3 x 10(2) CFU. The correlation coefficient was 0.89.
|
| 巻・号 |
63(4)
|
| ページ |
534-8
|
| 公開日 |
2000-4-1
|
| DOI |
10.4315/0362-028x-63.4.534
|
| PMID |
10772221
|
| MeSH |
Antibodies, Monoclonal
Antibody Specificity
Bacterial Toxins / isolation & purification*
Enterotoxins / isolation & purification*
Enzyme-Linked Immunosorbent Assay
Escherichia coli*
Filtration
Immunoenzyme Techniques
Luminol
Photons
Television
Water Microbiology*
|
| IF |
1.581
|
| 引用数 |
6
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
P3-X63-Ag8.653(RCB0146) |
