RRC ID 39866
Author Yamashita S, Akao T, Mizuki E, Saitoh H, Higuchi K, Park YS, Kim HS, Ohba M.
Title Characterization of the anti-cancer-cell parasporal proteins of a Bacillus thuringiensis isolate.
Journal Can. J. Microbiol.
Abstract An unusual activity, associated with non-insecticidal and non-haemolytic parasporal inclusion proteins of a Bacillus thuringiensis soil isolate, designated 89-T-26-17, was characterized. The parasporal inclusion of this isolate was bipyramidal, rounded at both ends, containing proteins of 180, 150, 120, 100, and 88 kDa. No homologies with the Cry and Cyt proteins of B. thuringiensis were detected based on N-terminal sequences. Proteolytic processing of the inclusion proteins by proteinase K, trypsin, and chymotrypsin produced a major protein of 64 kDa exhibiting cytocidal activity against human leukaemic T cells and uterus cervix cancer (HeLa) cells. The protease-activated proteins showed no cytotoxicity to normal T cells.
Volume 46(10)
Pages 913-9
Published 2000-10
PMID 11068678
MeSH Amino Acid Sequence Antineoplastic Agents / chemistry Antineoplastic Agents / metabolism Antineoplastic Agents / pharmacology* Bacillus thuringiensis / metabolism* Bacillus thuringiensis / ultrastructure Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Bacterial Proteins / pharmacology* Endopeptidase K / metabolism HeLa Cells Humans Inclusion Bodies / chemistry* Inclusion Bodies / ultrastructure Leukemia, T-Cell Molecular Sequence Data Spores, Bacterial Tumor Cells, Cultured
IF 1.243
Times Cited 26
Human and Animal Cells