RRC ID 39872
著者 Yamaguchi T, Takei N, Araki K, Ishii K, Nagano T, Ichikawa T, Kumanishi T, Nawa H.
タイトル Molecular characterization of a novel gamma-glutamyl transpeptidase homologue found in rat brain.
ジャーナル J Biochem
Abstract A cDNA clone for a novel homologue to gamma-glutamyl transpeptidase (gamma-GTP), termed GTPH, was isolated from a rat brain expression cDNA library using antisera against total brain synaptosomal fractions. The cloned GTPH consists of 641 amino acid residues (78 kDa) and exhibits structural similarity with a conventional type of gamma-GTP that is predominantly expressed in the liver: They share significant amino acid homology (33% identity, 73% similarity) spanning over the entire sequence. RNA analyses revealed that GTPH mRNA expression is found only in the nervous system, including all brain regions, eyes and peripheral ganglia, and increases during development. Endogenous GTPH protein is a membrane-bound glycoenzyme and migrates as 90-100 kDa in polyacrylamide gels. Taken together, GTPH is a novel form of a gamma-GTP-like molecule expressed exclusively in the nervous system.
巻・号 128(1)
ページ 101-6
公開日 2000-7-1
DOI 10.1093/oxfordjournals.jbchem.a022718
PMID 10876163
MeSH Amino Acid Sequence Animals Base Sequence Brain / enzymology* Gene Expression Regulation, Developmental Glycosylation Molecular Sequence Data Nervous System / embryology Nervous System / enzymology Rats gamma-Glutamyltransferase / genetics* gamma-Glutamyltransferase / immunology gamma-Glutamyltransferase / metabolism*
IF 2.476
引用数 4
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞 PC-12(RCB0009)