RRC ID 39903
Author Higashi T, Sasagawa T, Inoue M, Oka R, Shuangying L, Saijoh K.
Title Overexpression of latent transforming growth factor-beta 1 (TGF-beta 1) binding protein 1 (LTBP-1) in association with TGF-beta 1 in ovarian carcinoma.
Journal Jpn. J. Cancer Res.
Abstract Using the differential display method, latent transforming growth factor-beta 1 (TGF-beta 1) binding protein 1 (LTBP-1) mRNA was identified as one of the enriched mRNAs in ovarian carcinoma tissues after isolation of genes responsible for the development of ovarian cancer. Semi-quantitative reverse transcription (RT)-PCR analysis showed that expression of LTBP-1 and TGF-beta 1 mRNAs was much higher in both serous and mucinous adenocarcinomas than in their benign counterparts, including serous and mucinous cystadenomas and cystadenomas of low malignant potential (LMPs). Immunohistochemical analysis demonstrated that only proliferating benign adenoma cells were immunoreactive for both LTBP-1 and TGF-beta 1 proteins. In contrast, most serous and mucinous adenocarcinoma cells and their surrounding stroma were intensely immunoreactive for LTBP-1 and TGF-beta 1. LTBP-1 and TGF-beta 1 proteins, and their complex forms were identified in ovarian carcinoma cell lines and in their culture media by western blot analysis, suggesting these products were produced in ovarian carcinoma cells. RT-PCR analysis demonstrated that LTBP-1L, one of the LTBP-1 transcripts that has a strong activity in targeting the latent form of TGF-beta 1 to extracellular matrix (ECM), was predominantly expressed in ovarian carcinomas. Taken together, the results suggest that upregulation of LTBP-1 in ovarian carcinoma cells may have an important role in distributing TGF-beta1 in the stromal tissues surrounding carcinoma cells.
Volume 92(5)
Pages 506-15
Published 2001-5
DOI 10.1111/j.1349-7006.2001.tb01123.x
PMID 11376559
PMC PMC5926747
MeSH Adenocarcinoma, Mucinous / chemistry Adenocarcinoma, Mucinous / genetics Blotting, Western Carrier Proteins / analysis Carrier Proteins / genetics* Cystadenocarcinoma, Serous / chemistry Cystadenocarcinoma, Serous / genetics Cystadenoma, Mucinous / chemistry Cystadenoma, Mucinous / genetics Female Gene Expression* Humans Immunohistochemistry Intracellular Signaling Peptides and Proteins* Latent TGF-beta Binding Proteins Ovarian Neoplasms / chemistry Ovarian Neoplasms / genetics* RNA, Messenger / analysis Reverse Transcriptase Polymerase Chain Reaction Transforming Growth Factor beta / analysis Transforming Growth Factor beta / genetics*
Times Cited 21
Human and Animal Cells