RRC ID 39904
著者 Kusumi T, Nishi T, Tanaka M, Tsuchida S, Kudo H.
タイトル A murine osteosarcoma cell line with a potential to develop ossification upon transplantation.
ジャーナル Jpn J Cancer Res
Abstract An osteosarcoma cell line has been established from a soft tissue tumor that occurred spontaneously in a BALB / c mouse. This cell line showed ossification when transplanted into syngeneic mice. To examine the mechanism of bone formation, the expression of mRNAs for osteoblastic and chondroblastic markers and factors associated with ossification has been investigated. In culture, the cells exhibited a spindle shape in the growth phase, but had a polygonal shape in the stationary phase. Reverse transcription-polymerase chain reaction analysis showed that the cells expressed mRNAs for pro-alpha1(I) chain of type I collagen, alkaline phosphatase, osteopontin, osteocalcin, and core binding factor alpha1, suggesting differentiation into the stage of osteoblasts during the stationary phase. After transplantation, histological examination revealed small foci of pale blue material and basophilic networks that were scattered in the tumor tissues at one week. The former stained positive with alcian blue, suggesting a chondroid matrix. Pro-alpha1(II) chain of type II collagen mRNA was expressed at one week. A large part of tumors at two and three weeks consisted of basophilic networks, which stained positive via von Kossa's method, indicating a calcified woven bone. In situ hybridization analysis showed strong expression of osteopontin and osteocalcin mRNAs in tumor cells surrounding the bone matrix. Bone morphogenetic protein-6 and -7 mRNAs were detected in transplanted tumors, but not in cultured cells. These results suggest that the cell line has the properties of an osteoblastic lineage when cultured in vitro and has an ossifying ability through endochondral bone formation processes when transplanted in vivo.
巻・号 92(6)
ページ 649-58
公開日 2001-6-1
DOI 10.1111/j.1349-7006.2001.tb01144.x
PMID 11429054
PMC PMC5926767
MeSH Alkaline Phosphatase / biosynthesis Animals Bone Development / physiology Cell Culture Techniques / methods Core Binding Factor alpha Subunits DNA-Binding Proteins / biosynthesis Extremities / diagnostic imaging Female In Situ Hybridization Mice Mice, Inbred BALB C Neoplasm Transplantation Osteocalcin / biosynthesis Osteopontin Osteosarcoma / pathology* Procollagen / biosynthesis RNA, Messenger / metabolism Radiography Reverse Transcriptase Polymerase Chain Reaction Sialoglycoproteins / biosynthesis Soft Tissue Neoplasms / pathology Time Factors Transcription Factors / biosynthesis Transplantation* Tumor Cells, Cultured*
引用数 5
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)