論文 - 詳細
| RRC ID | 39913 |
|---|---|
| 著者 | Sakiyama H, Masuda R, Inoue N, Yamamoto K, Kuriiwa K, Nakagawa K, Yoshida K. |
| タイトル | Establishment and characterization of macrophage-like cell lines expressing osteoclast-specific markers. |
| ジャーナル | J Bone Miner Metab |
| Abstract |
Osteoclasts differentiate from hematopoietic precursors of the monocyte/macrophage lineage to mononuclear preosteoclasts and multinuclear mature osteoclasts. In the present study, we report on the establishment of macrophage like cell lines from mouse bone marrow by coculturing bone marrow cells with mouse chondrocytes. Isolated clones (MLC-6 and MLC-7 cells) expressed fully differentiated osteoclast markers, such as calcitonin receptors, vitronectin receptors, tartrate-resistant acid phosphatase and vacuolar H+ -ATPase, in the absence of osteoclast differentiation factor/osteoprotegerin ligand/RANKL/TRANCE, which was essential for osteoclast differentiation. Most clones also maintained expression of a macrophage-associated protein, namely non-specific esterase. Both MLC-6 and MLC-7 cells released cathepsin K into the culture medium. Both clones resolved dentine slices when cocultured with the osteoblast cell line ST2. The cloned cell lines are considered to be useful tools in the study of osteoclast differentiation. |
| 巻・号 | 19(4) |
| ページ | 220-7 |
| 公開日 | 2001-1-1 |
| DOI | 10.1007/s007740170024 |
| PMID | 11448014 |
| MeSH | Animals Bacterial Proteins* Base Sequence Biomarkers Cell Differentiation Cell Line Coculture Techniques DNA Primers / genetics Gene Expression Glycoproteins / genetics Immunohistochemistry Macrophages / cytology* Macrophages / metabolism* Matrix Metalloproteinase 9 / genetics Mice Microscopy, Electron, Scanning Osteoclasts / cytology* Osteoclasts / metabolism* Osteoprotegerin RNA-Binding Proteins / genetics Receptors, Calcitonin / genetics Receptors, Cytoplasmic and Nuclear / genetics Receptors, Tumor Necrosis Factor Receptors, Vitronectin / genetics Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / genetics Vacuolar Proton-Translocating ATPases / metabolism |
| IF | 2.297 |
| 引用数 | 25 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ST2(RCB0224) MC3T3-E1(RCB1126) |