| RRC ID |
39925
|
| Author |
Wasa M, Wang HS, Tazuke Y, Okada A.
|
| Title |
Insulin-like growth factor-I stimulates amino acid transport in a glutamine-deprived human neuroblastoma cell line.
|
| Journal |
Biochim Biophys Acta
|
| Abstract |
It is still unknown how insulin-like growth factor-I (IGF-I) regulates cancer cell growth in the condition of the limited availability of key nutrients, such as glutamine. We investigated the effects of IGF-I on cell growth and amino acid transport in a glutamine-deprived human neuroblastoma cell line, SK-N-SH. Cell growth was measured, and 3H-labeled amino acid transport was assayed after treatment with or without IGF-I (50 ng/ml) in 2 mM (control) and 100 microM glutamine concentrations. Cell growth rates were dependent on glutamine concentrations. IGF-I stimulated cell growth in both 2 mM and 100 microM glutamine. IGF-I stimulated glutamine transport in 100 microM glutamine with the mechanism of increasing carrier Vmax, but had no effect in 2 mM glutamine. IGF-I also stimulated leucine, glutamate and 2-(methylamino)isobutyric acid transport in 100 microM glutamine. There were significant increases in [3H]thymidine and [3H]leucine incorporation in IGF-I-treated cells in both 2 mM and 100 microM glutamine. These data suggest that IGF-I stimulates cell growth by increasing amino acid transport in the condition of low glutamine levels in a human neuroblastoma cell line. This mechanism may allow to maintain cell growth even in nutrient-deprived tumor tissues.
|
| Volume |
1525(1-2)
|
| Pages |
118-24
|
| Published |
2001-2-16
|
| DOI |
10.1016/s0304-4165(00)00178-1
|
| PII |
S0304-4165(00)00178-1
|
| PMID |
11342260
|
| MeSH |
Amino Acids / metabolism*
Biological Transport, Active / drug effects
Cell Division / drug effects
Cycloheximide / pharmacology
DNA, Neoplasm / biosynthesis
Glutamic Acid / metabolism
Glutamine / deficiency
Glutamine / metabolism*
Humans
Insulin-Like Growth Factor I / pharmacology*
Leucine / metabolism
Neoplasm Proteins / biosynthesis
Neuroblastoma / metabolism
Neuroblastoma / pathology
Protein Synthesis Inhibitors / pharmacology
Tumor Cells, Cultured
beta-Alanine / analogs & derivatives*
beta-Alanine / metabolism
|
| IF |
3.411
|
| Times Cited |
6
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
SK-N-SH(RCB0426) |
