論文 - 詳細
| RRC ID | 39938 |
|---|---|
| 著者 | Takahashi F, Takahashi K, Okazaki T, Maeda K, Ienaga H, Maeda M, Kon S, Uede T, Fukuchi Y. |
| タイトル | Role of osteopontin in the pathogenesis of bleomycin-induced pulmonary fibrosis. |
| ジャーナル | Am J Respir Cell Mol Biol |
| Abstract |
Pulmonary fibrosis is initiated by migration, adhesion, and proliferation of fibroblasts. Osteopontin (OPN) is one of the cytokines produced by activated macrophages and mediates various functions, including cell attachment and migration, by interacting with alphav integrin. In this study, we have investigated the role of OPN in the pathogenesis of pulmonary fibrosis. We developed a mouse model for pulmonary fibrosis by intratracheal instillation of bleomycin (BLM). OPN was strongly expressed in alveolar macrophages accumulating in the fibrotic area of the lung. OPN messenger RNA (mRNA) in the lung was notably induced by BLM instillation, and the development of the fibrotic process was associated with an increase in the expression of OPN mRNA and protein. In vitro, recombinant OPN enhanced migration, adhesion, and platelet-derived growth factor (PDGF)-mediated DNA synthesis of murine fibroblast cell line NIH3T3. These effects of OPN on fibroblasts were significantly suppressed by addition of antimouse alphav integrin monoclonal antibody (RMV-7). Furthermore, treatment of mice with RMV-7 repressed the extent of pulmonary fibrosis in this model. Conclusively, these data suggest that OPN produced by alveolar macrophages functions as a fibrogenic cytokine that promotes migration, adhesion, and proliferation of fibroblasts in the development of BLM-induced pulmonary fibrosis. |
| 巻・号 | 24(3) |
| ページ | 264-71 |
| 公開日 | 2001-3-1 |
| DOI | 10.1165/ajrcmb.24.3.4293 |
| PMID | 11245625 |
| MeSH | Animals Bleomycin / toxicity* Cell Adhesion / physiology Cell Line Cell Movement / physiology Cytokines / physiology Disease Models, Animal Hydroxyproline / analysis Inflammation Lung / pathology Lung / physiology Lung / physiopathology Macrophages, Alveolar / pathology Macrophages, Alveolar / physiology* Male Mice Mice, Inbred ICR Osteopontin Pulmonary Fibrosis / chemically induced Pulmonary Fibrosis / pathology Pulmonary Fibrosis / physiopathology* RNA, Messenger / genetics Recombinant Fusion Proteins / metabolism Sialoglycoproteins / analysis Sialoglycoproteins / genetics Sialoglycoproteins / physiology* Time Factors Transcription, Genetic* |
| IF | 5.373 |
| 引用数 | 103 |
| WOS 分野 | RESPIRATORY SYSTEM BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) WI-38 |