RRC ID 3995
Author Kwon C, Neu C, Pajonk S, Yun HS, Lipka U, Humphry M, Bau S, Straus M, Kwaaitaal M, Rampelt H, El Kasmi F, Jürgens G, Parker J, Panstruga R, Lipka V, Schulze-Lefert P.
Title Co-option of a default secretory pathway for plant immune responses.
Journal Nature
Abstract Cell-autonomous immunity is widespread in plant-fungus interactions and terminates fungal pathogenesis either at the cell surface or after pathogen entry. Although post-invasive resistance responses typically coincide with a self-contained cell death of plant cells undergoing attack by parasites, these cells survive pre-invasive defence. Mutational analysis in Arabidopsis identified PEN1 syntaxin as one component of two pre-invasive resistance pathways against ascomycete powdery mildew fungi. Here we show that plasma-membrane-resident PEN1 promiscuously forms SDS-resistant soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) complexes together with the SNAP33 adaptor and a subset of vesicle-associated membrane proteins (VAMPs). PEN1-dependent disease resistance acts in vivo mainly through two functionally redundant VAMP72 subfamily members, VAMP721 and VAMP722. Unexpectedly, the same two VAMP proteins also operate redundantly in a default secretory pathway, suggesting dual functions in separate biological processes owing to evolutionary co-option of the default pathway for plant immunity. The disease resistance function of the secretory PEN1-SNAP33-VAMP721/722 complex and the pathogen-induced subcellular dynamics of its components are mechanistically reminiscent of immunological synapse formation in vertebrates, enabling execution of immune responses through focal secretion.
Volume 451(7180)
Pages 835-40
Published 2008-2-14
DOI 10.1038/nature06545
PII nature06545
PMID 18273019
MeSH ATP-Binding Cassette Transporters / genetics ATP-Binding Cassette Transporters / metabolism Arabidopsis / genetics Arabidopsis / immunology* Arabidopsis / microbiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Ascomycota / physiology N-Glycosyl Hydrolases / genetics N-Glycosyl Hydrolases / metabolism Qa-SNARE Proteins / genetics Qa-SNARE Proteins / metabolism SNARE Proteins / genetics SNARE Proteins / metabolism
IF 42.779
Times Cited 276
Arabidopsis / Cultured plant cells, genes pda08554 pda09643 pda07879