RRC ID 39959
Author Nadano D, Aoki C, Yoshinaka T, Irie S, Sato TA.
Title Electrophoretic characterization of ribosomal subunits and proteins in apoptosis: specific downregulation of S11 in staurosporine-treated human breast carcinoma cells.
Journal Biochemistry
Abstract Stimulation of death receptors (Fas on human T-cell leukemia Jurkat cells and tumor necrosis factor receptor-1 on human monoblastic leukemia U937 cells) triggers the specific degradation of 28S ribosomal RNA, and this process may contribute to cell death through the inhibition of protein synthesis. We have developed an analytical method using a polyacrylamide-agarose composite gel to evaluate ribosomal subunits in apoptotic cells (human breast carcinoma MCF-7 cells treated with staurosporine and human 293T cells irradiated with ultraviolet light were used in addition to the two apoptosis systems described above). No alterations were detected by this method, suggesting that apoptosis, including the process of ribosomal RNA degradation, does not cause fragmentation or extensive conformational changes in the ribosome. We also examined the status of 21 different ribosomal proteins in apoptotic cells by immunoblotting with polyclonal antibodies. S11 was specifically downregulated in apoptotic MCF-7 cells and in other apoptotic breast carcinoma cells. Previous studies have shown that S11 is heterogeneously expressed in cancer cells. Taken together, it appears that particular intracellular environments regulate the expression of S11 protein. However, the mechanism by which this process is modulated is as yet unknown. Furthermore, we have demonstrated that our composite gel electrophoresis system can efficiently detect ubiquitination of ribosomal subunits.
Volume 40(50)
Pages 15184-93
Published 2001-12-18
DOI 10.1021/bi0108397
PII bi0108397
PMID 11735401
MeSH Amino Acid Sequence Animals Apoptosis Breast Neoplasms / drug therapy* Breast Neoplasms / genetics Breast Neoplasms / metabolism* Breast Neoplasms / pathology Down-Regulation / drug effects Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Female Humans Jurkat Cells Male Molecular Sequence Data Rats Rats, Sprague-Dawley Ribosomal Proteins / genetics Ribosomal Proteins / metabolism* Ribosomes / drug effects Ribosomes / metabolism Staurosporine / pharmacology* Tumor Cells, Cultured U937 Cells Ubiquitin / metabolism
IF 2.865
Times Cited 21
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells NEC8(RCB0489)