RRC ID 39962
著者 Sasaki M, Ito T, Kashima M, Fukui S, Izumiyama N, Watanabe A, Sano M, Fujiwara Y, Miura M.
タイトル Erythromycin and clarithromycin modulation of growth factor-induced expression of heparanase mRNA on human lung cancer cells in vitro.
ジャーナル Mediators Inflamm
Abstract Heparanase activity is correlated with the metastatic potential of several cancer cells and is a key enzyme in the breakdown of tissue barriers. It is also involved in the regulation of growth factor and cytokine activity. However, little is known about the factors that induce heparanase in cancer cells. We investigated the effect of three growth factors, platelet-derived growth factor (PDGF), hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF), on heparanase mRNA induction in lung cancer cells in vitro. In addition, we examined the effect of erythromycin (EM) and clarithromycin (CAM), which are 14-membered ring macrolide antibiotics that act as biological response modifiers, on the expression of heparanase mRNA induced by growth factors. PDGF, HGF and bFGF stimulated cell migration activity and enhanced the expression of heparanase mRNA in the human lung adenocarcinoma cell line A549. Via different mechanisms, EM and CAM modulate the induction by these factors of heparanase mRNA expression on A549 cells. EM also significantly suppressed A549 cell migration induced by PDGF and HGF, and CAM significantly suppressed A549cell migration induced by bFGF. The results suggest that the growth factors PDGF, HGF and bFGF are important inducers of heparanase in potentially invasive and metastatic cancer cells. The suppressive effect of heparanase mRNA expression by EM and CAM may have interestingtherapeutic applications in the prevention of metastasis.
巻・号 10(5)
ページ 259-67
公開日 2001-10-1
DOI 10.1080/09629350120093731
PMID 11759110
PMC PMC1781717
MeSH Anti-Bacterial Agents / pharmacology Cell Movement / drug effects Clarithromycin / pharmacology* Erythromycin / pharmacology* Fibroblast Growth Factor 2 / pharmacology Gene Expression / drug effects Glucuronidase / genetics* Hepatocyte Growth Factor / pharmacology Humans Immunologic Factors / pharmacology Lung Neoplasms / enzymology Lung Neoplasms / genetics Lung Neoplasms / pathology Lung Neoplasms / secondary Platelet-Derived Growth Factor / pharmacology RNA, Messenger / genetics* RNA, Messenger / metabolism* Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured
IF 3.758
引用数 12
WOS 分野 IMMUNOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 Human lung fibroblasts