| RRC ID |
39969
|
| 著者 |
Masuoka J, Shiraishi T, Ichinose M, Mineta T, Tabuchi K.
|
| タイトル |
Expression of ICAD-l and ICAD-S in human brain tumor and its cleavage upon activation of apoptosis by anti-Fas antibody.
|
| ジャーナル |
Jpn J Cancer Res
|
| Abstract |
ICAD / DFF is a downstream molecule of caspases, participating in nuclear DNA fragmentation during apoptosis. ICAD / DFF binds CAD / DFF40 and inhibits its DNase activity. ICAD / DFF has two alternative isoforms, long isoform (ICAD-L / DFF45) and short isoform (ICAD-S / DFF35). We have studied the presence and functional status of ICAD / DFF in human glioma cell lines. All cell lines tested expressed both ICAD-L and ICAD-S. When the cultured glioma cells were exposed to anti-Fas antibody, these isoforms were degraded prior to the fragmentation of the nuclear DNA, indicating that the ICAD / DFF expressed in cultured glioma cells was potentially functional. In primary brain tumors and normal brain tissues, there was a difference in the expression level between ICAD-L and ICAD-S. In glioblastomas, ICAD-S was more abundant than ICAD-L. In contrast, ICAD-L was more abundant than ICAD-S in medulloblastomas. The present findings suggest that primary brain tumors and normal brain constitutively express ICAD / DFF, and that there is a difference between the expression levels of ICAD-L and ICAD-S.
|
| 巻・号 |
92(7)
|
| ページ |
806-12
|
| 公開日 |
2001-7-1
|
| DOI |
10.1111/j.1349-7006.2001.tb01165.x
|
| PMID |
11473733
|
| PMC |
PMC5926781
|
| MeSH |
Antibodies, Monoclonal / pharmacology*
Antibodies, Monoclonal, Murine-Derived
Apoptosis / immunology
Apoptosis / physiology*
Apoptosis Regulatory Proteins
Blotting, Western
Brain Neoplasms / metabolism*
Brain Neoplasms / pathology
Glioma / metabolism*
Glioma / pathology
Humans
Protein Biosynthesis*
Protein Isoforms
Proteins / metabolism
Tumor Cells, Cultured
fas Receptor / immunology
fas Receptor / physiology*
|
| 引用数 |
7
|
| リソース情報 |
| ヒト・動物細胞 |
U251(RCB0461) |
