RRC ID 4000
Author Zhang X, Liu S, Takano T.
Title Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana.
Journal Biotechnol. Lett.
Abstract Mitochondrial F(1)F(0)-ATPase is a key enzyme in plant metabolism, providing cells with ATP that uses the transmembrane electrochemical proton gradient to drive synthesis of ATP. A 6 kDa protein (At3g46430) has been previously purified from Arabidopsis thaliana mitochondrial F(1)F(0)-ATPase. In this study, the gene (AtMtATP6; GenBank accession no. AK117680) encoding this protein was isolated from Arabidopsis and characterized. Northern blot analyses showed that the expression of AtMtATP6 gene in Arabidopsis suspension-cultured cells was induced by several abiotic stresses from salts, drought, and cold. Over-expression of AtMtATP6 gene in transgenic yeast and Arabidopsis plants increased the resistance to salts, drought, oxidative and cold stresses. Taken together, our data raise the possibility that induction of the F(1)F(0)-ATPase plays a role in stress tolerance.
Volume 30(7)
Pages 1289-94
Published 2008-7
DOI 10.1007/s10529-008-9685-6
PMID 18338219
MeSH Arabidopsis / cytology Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / biosynthesis* Arabidopsis Proteins / genetics Cold Temperature Membrane Proteins / biosynthesis* Membrane Proteins / genetics Mitochondrial Proteins / biosynthesis* Mitochondrial Proteins / genetics Peroxins Proton-Translocating ATPases / genetics Proton-Translocating ATPases / metabolism* Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Salts / pharmacology
IF 1.846
Times Cited 28
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes rpc00008