| RRC ID |
4001
|
| 著者 |
Pavón LR, Lundh F, Lundin B, Mishra A, Persson BL, Spetea C.
|
| タイトル |
Arabidopsis ANTR1 is a thylakoid Na+-dependent phosphate transporter: functional characterization in Escherichia coli.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
In this study, the putative anion transporter 1 (ANTR1) from Arabidopsis thaliana was shown to be localized to the chloroplast thylakoid membrane by Western blotting with two different peptide-specific antibodies. ANTR1 is homologous to the type I of mammalian Na+-dependent inorganic phosphate (Pi) transporters. The function of ANTR1 as a Na+-dependent Pi transporter was demonstrated by heterologous expression and uptake of radioactive Pi into Escherichia coli cells. The expression of ANTR1 conferred increased growth rates to the transformed cells and stimulated Pi uptake in a pH- and Na+-dependent manner as compared with the control cells. Among various tested effectors, Pi was the preferred substrate. Although it competed with the uptake of Pi, glutamate was not transported by ANTR1 into E. coli. In relation to its function as a Pi transporter, several physiological roles for ANTR1 in the thylakoid membrane are proposed, such as export of Pi produced during nucleotide metabolism in the thylakoid lumen back to the chloroplast stroma and balance of the trans-thylakoid H+ electrochemical gradient storage.
|
| 巻・号 |
283(20)
|
| ページ |
13520-7
|
| 公開日 |
2008-5-16
|
| DOI |
10.1074/jbc.M709371200
|
| PII |
S0021-9258(20)71584-X
|
| PMID |
18353780
|
| MeSH |
Amino Acid Sequence
Arabidopsis / genetics*
Arabidopsis / metabolism
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / physiology*
Escherichia coli / metabolism*
Gene Expression Regulation
Gene Expression Regulation, Plant
Hydrogen-Ion Concentration
Membrane Transport Proteins / metabolism
Models, Biological
Molecular Sequence Data
Phosphate Transport Proteins / chemistry
Phosphate Transport Proteins / metabolism*
Phosphate Transport Proteins / physiology
Plant Physiological Phenomena
Sequence Homology, Amino Acid
Sodium / metabolism*
Thylakoids / metabolism*
|
| IF |
4.238
|
| 引用数 |
44
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda05103 |
