RRC ID 4001
Author Pavón LR, Lundh F, Lundin B, Mishra A, Persson BL, Spetea C.
Title Arabidopsis ANTR1 is a thylakoid Na+-dependent phosphate transporter: functional characterization in Escherichia coli.
Journal J. Biol. Chem.
Abstract In this study, the putative anion transporter 1 (ANTR1) from Arabidopsis thaliana was shown to be localized to the chloroplast thylakoid membrane by Western blotting with two different peptide-specific antibodies. ANTR1 is homologous to the type I of mammalian Na+-dependent inorganic phosphate (Pi) transporters. The function of ANTR1 as a Na+-dependent Pi transporter was demonstrated by heterologous expression and uptake of radioactive Pi into Escherichia coli cells. The expression of ANTR1 conferred increased growth rates to the transformed cells and stimulated Pi uptake in a pH- and Na+-dependent manner as compared with the control cells. Among various tested effectors, Pi was the preferred substrate. Although it competed with the uptake of Pi, glutamate was not transported by ANTR1 into E. coli. In relation to its function as a Pi transporter, several physiological roles for ANTR1 in the thylakoid membrane are proposed, such as export of Pi produced during nucleotide metabolism in the thylakoid lumen back to the chloroplast stroma and balance of the trans-thylakoid H+ electrochemical gradient storage.
Volume 283(20)
Pages 13520-7
Published 2008-5-16
DOI 10.1074/jbc.M709371200
PII M709371200
PMID 18353780
MeSH Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics* Arabidopsis Proteins / physiology* Escherichia coli / metabolism* Gene Expression Regulation Gene Expression Regulation, Plant Hydrogen-Ion Concentration Membrane Transport Proteins / metabolism Models, Biological Molecular Sequence Data Phosphate Transport Proteins / chemistry Phosphate Transport Proteins / metabolism* Phosphate Transport Proteins / physiology Plant Physiological Phenomena Sequence Homology, Amino Acid Sodium / metabolism* Thylakoids / metabolism*
IF 4.011
Times Cited 29
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda05103