論文 - 詳細
| RRC ID | 40022 |
|---|---|
| 著者 | Auer TO, Xiao T, Bercier V, Gebhardt C, Duroure K, Concordet JP, Wyart C, Suster M, Kawakami K, Wittbrodt J, Baier H, Del Bene F. |
| タイトル | Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. |
| ジャーナル | Elife |
| Abstract |
Development and function of highly polarized cells such as neurons depend on microtubule-associated intracellular transport, but little is known about contributions of specific molecular motors to the establishment of synaptic connections. In this study, we investigated the function of the Kinesin I heavy chain Kif5aa during retinotectal circuit formation in zebrafish. Targeted disruption of Kif5aa does not affect retinal ganglion cell differentiation, and retinal axons reach their topographically correct targets in the tectum, albeit with a delay. In vivo dynamic imaging showed that anterograde transport of mitochondria is impaired, as is synaptic transmission. Strikingly, disruption of presynaptic activity elicits upregulation of Neurotrophin-3 (Ntf3) in postsynaptic tectal cells. This in turn promotes exuberant branching of retinal axons by signaling through the TrkC receptor (Ntrk3). Thus, our study has uncovered an activity-dependent, retrograde signaling pathway that homeostatically controls axonal branching. |
| 巻・号 | 4 |
| 公開日 | 2015-6-15 |
| DOI | 10.7554/eLife.05061 |
| PMID | 26076409 |
| PMC | PMC4467164 |
| MeSH | Animals Animals, Genetically Modified Axons / physiology* Biological Transport / physiology Blotting, Western Cell Polarity / physiology* Chromosome Mapping Cloning, Molecular DNA Primers / genetics Genotype Immunohistochemistry In Situ Hybridization Kinesins / genetics Kinesins / metabolism* Microscopy, Confocal Microscopy, Electron, Transmission Mitochondria / metabolism Neurogenesis / physiology* Neurotrophin 3 / metabolism* Real-Time Polymerase Chain Reaction Signal Transduction / physiology* Time-Lapse Imaging Zebrafish / embryology* Zebrafish Proteins / metabolism* |
| IF | 7.08 |
| 引用数 | 19 |
| WOS 分野 | BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | gSA2AzGFF49A |