Reference - Detail
| RRC ID | 40036 |
|---|---|
| Author | Bratic A, Clemente P, Calvo-Garrido J, Maffezzini C, Felser A, Wibom R, Wedell A, Freyer C, Wredenberg A. |
| Title | Mitochondrial Polyadenylation Is a One-Step Process Required for mRNA Integrity and tRNA Maturation. |
| Journal | PLoS Genet |
| Abstract |
Polyadenylation has well characterised roles in RNA turnover and translation in a variety of biological systems. While polyadenylation on mitochondrial transcripts has been suggested to be a two-step process required to complete translational stop codons, its involvement in mitochondrial RNA turnover is less well understood. We studied knockdown and knockout models of the mitochondrial poly(A) polymerase (MTPAP) in Drosophila melanogaster and demonstrate that polyadenylation of mitochondrial mRNAs is exclusively performed by MTPAP. Further, our results show that mitochondrial polyadenylation does not regulate mRNA stability but protects the 3' terminal integrity, and that despite a lack of functioning 3' ends, these trimmed transcripts are translated, suggesting that polyadenylation is not required for mitochondrial translation. Additionally, loss of MTPAP leads to reduced steady-state levels and disturbed maturation of tRNACys, indicating that polyadenylation in mitochondria might be important for the stability and maturation of specific tRNAs. |
| Volume | 12(5) |
| Pages | e1006028 |
| Published | 2016-5-1 |
| DOI | 10.1371/journal.pgen.1006028 |
| PII | PGENETICS-D-16-00334 |
| PMID | 27176048 |
| PMC | PMC4866704 |
| MeSH | Animals Codon, Terminator Drosophila melanogaster / genetics* Gene Knockdown Techniques Mitochondria / genetics Mitochondrial Proteins / biosynthesis Mitochondrial Proteins / genetics Polyadenylation / genetics* Protein Biosynthesis / genetics* RNA, Messenger / genetics* RNA, Mitochondrial RNA, Transfer / genetics |
| IF | 5.175 |
| Times Cited | 22 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 11418R-1 |