Reference - Detail
| RRC ID | 4006 |
|---|---|
| Author | Pujol C, Bailly M, Kern D, Maréchal-Drouard L, Becker H, Duchêne AM. |
| Title | Dual-targeted tRNA-dependent amidotransferase ensures both mitochondrial and chloroplastic Gln-tRNAGln synthesis in plants. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Aminoacyl-tRNAs are generally formed by direct attachment of an amino acid to tRNAs by aminoacyl-tRNA synthetases, but Gln-tRNA is an exception to this rule. Gln-tRNA(Gln) is formed by this direct pathway in the eukaryotic cytosol and in protists or fungi mitochondria but is formed by an indirect transamidation pathway in most of bacteria, archaea, and chloroplasts. We show here that the formation of Gln-tRNA(Gln) is also achieved by the indirect pathway in plant mitochondria. The mitochondrial-encoded tRNA(Gln), which is the only tRNA(Gln) present in mitochondria, is first charged with glutamate by a nondiscriminating GluRS, then is converted into Gln-tRNA(Gln) by a tRNA-dependent amidotransferase (AdT). The three subunits GatA, GatB, and GatC are imported into mitochondria and assemble into a functional GatCAB AdT. Moreover, the mitochondrial pathway of Gln-tRNA(Gln) formation is shared with chloroplasts as both the GluRS, and the three AdT subunits are dual-imported into mitochondria and chloroplasts. |
| Volume | 105(17) |
| Pages | 6481-5 |
| Published | 2008-4-29 |
| DOI | 10.1073/pnas.0712299105 |
| PII | 0712299105 |
| PMID | 18441100 |
| PMC | PMC2359783 |
| MeSH | Arabidopsis / enzymology* Cell Extracts Chloroplasts / enzymology* Cytosol / enzymology Glutamate-tRNA Ligase / metabolism Glutamine / biosynthesis* Mitochondria / enzymology* Nitrogenous Group Transferases / metabolism* Protein Subunits / metabolism Protein Transport RNA, Transfer, Amino Acyl / biosynthesis* Solanum tuberosum / enzymology* |
| IF | 9.412 |
| Times Cited | 43 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda01467 pda05658 |