RRC ID |
40070
|
Author |
Condas LA, Ribeiro MG, Muro MD, de Vargas AP, Matsuzawa T, Yazawa K, Siqueira AK, Salerno T, Lara GH, Risseti RM, Ferreira KS, Gonoi T.
|
Title |
MOLECULAR IDENTIFICATION AND ANTIMICROBIAL RESISTANCE PATTERN OF SEVEN CLINICAL ISOLATES OF Nocardia spp. IN BRAZIL.
|
Journal |
Rev Inst Med Trop Sao Paulo
|
Abstract |
Nocardia is a ubiquitous microorganism related to pyogranulomatous infection, which is difficult to treat in humans and animals. The occurrence of the disease is on the rise in many countries due to an increase in immunosuppressive diseases and treatments. This report of cases from Brazil presents the genotypic characterization and the antimicrobial susceptibility pattern using the disk-diffusion method and inhibitory minimal concentration with E-test® strips. In summary, this report focuses on infections in young adult men, of which three cases were cutaneous, two pulmonary, one neurological and one systemic. The pulmonary, neurological and systemic cases were attributed to immunosuppressive diseases or treatments. Sequencing analysis of the 16S rRNA segments (1491 bp) identified four isolates of Nocardia farcinica, two isolates of Nocardia nova and one isolate of Nocardia asiatica. N. farcinica was involved in two cutaneous, one systemic and other pulmonary cases; N. nova was involved in one neurological and one pulmonary case; and Nocardia asiatica in one cutaneous case. The disk-diffusion antimicrobial susceptibility test showed that the most effective antimicrobials were amikacin (100%), amoxicillin/clavulanate (100%), cephalexin (100%) and ceftiofur (100%), while isolates had presented most resistance to gentamicin (43%), sulfamethoxazole/trimethoprim (43%) and ampicillin (29%). However, on the inhibitory minimal concentration test (MIC test), only one of the four isolates of Nocardia farcinica was resistant to sulfamethoxazole/trimethoprim.
|
Volume |
57(3)
|
Pages |
251-6
|
Published |
2015-1-1
|
DOI |
10.1590/S0036-46652015000300012
|
PII |
S0036-46652015000300251
|
PMID |
26200967
|
PMC |
PMC4544251
|
MeSH |
Adult
Animals
Anti-Bacterial Agents / pharmacology*
Bacterial Typing Techniques
Brazil
DNA, Bacterial / genetics
Disk Diffusion Antimicrobial Tests
Humans
Male
Nocardia / classification
Nocardia / genetics*
Nocardia / isolation & purification
Nocardia Infections / microbiology*
RNA, Ribosomal, 16S / genetics
|
IF |
1.46
|
Times Cited |
2
|
WOS Category
|
TROPICAL MEDICINE
|
Resource |
Pathogenic microorganisms |
IFM 11096
IFM 11099
IFM 11100
IFM 11128
IFM 11231
IFM 11232
IFM 11285 |