RRC ID 40077
Author Kopecká M, Yamaguchi M, Kawamoto S.
Title Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae.
Journal Microbiology (Reading, Engl.)
Abstract Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Bürker chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance of actin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing β-1,3-glucan microfibrils. The LA-inhibited cells lysed. Actin cables and patches were required for bud formation and bud growth. In addition, actin patches were required for the formation of β-1,3-glucan microfibrils in the bud cell wall. LA has fungistatic, fungicidal and fungilytic effects on the budding yeast Saccharomyces cerevisiae.
Volume 161(7)
Pages 1348-55
Published 2015-7
DOI 10.1099/mic.0.000091
PII mic.0.000091
PMID 25858300
MeSH Actins / antagonists & inhibitors* Antifungal Agents / pharmacology* Bridged Bicyclo Compounds, Heterocyclic / pharmacology* Colony Count, Microbial Microbial Viability / drug effects Microscopy Saccharomyces cerevisiae / cytology Saccharomyces cerevisiae / drug effects* Saccharomyces cerevisiae / physiology Saccharomycetales / cytology Saccharomycetales / drug effects* Saccharomycetales / physiology Thiazolidines / pharmacology*
IF 1.866
Times Cited 2
Pathogenic microorganisms ?