| RRC ID |
40084
|
| Author |
Sakai K, Komaki H, Gonoi T.
|
| Title |
Identification and Functional Analysis of the Nocardithiocin Gene Cluster in Nocardia pseudobrasiliensis.
|
| Journal |
PLoS One
|
| Abstract |
Nocardithiocin is a thiopeptide compound isolated from the opportunistic pathogen Nocardia pseudobrasiliensis. It shows a strong activity against acid-fast bacteria and is also active against rifampicin-resistant Mycobacterium tuberculosis. Here, we report the identification of the nocardithiocin gene cluster in N. pseudobrasiliensis IFM 0761 based on conserved thiopeptide biosynthesis gene sequence and the whole genome sequence. The predicted gene cluster was confirmed by gene disruption and complementation. As expected, strains containing the disrupted gene did not produce nocardithiocin while gene complementation restored nocardithiocin production in these strains. The predicted cluster was further analyzed using RNA-seq which showed that the nocardithiocin gene cluster contains 12 genes within a 15.2-kb region. This finding will promote the improvement of nocardithiocin productivity and its derivatives production.
|
| Volume |
10(11)
|
| Pages |
e0143264
|
| Published |
2015-1-1
|
| DOI |
10.1371/journal.pone.0143264
|
| PII |
PONE-D-15-27385
|
| PMID |
26588225
|
| PMC |
PMC4654471
|
| MeSH |
Anti-Bacterial Agents / biosynthesis
DNA Primers
Genetic Complementation Test
Genome, Bacterial
Mass Spectrometry
Multigene Family*
Mycobacterium / drug effects
Nocardia / genetics*
Nocardia / metabolism
Peptides / chemistry
Peptides, Cyclic / genetics*
Peptides, Cyclic / metabolism*
Sequence Analysis, DNA
Sequence Analysis, RNA
Species Specificity
Spectrophotometry, Ultraviolet
Thiazoles / metabolism*
|
| IF |
2.74
|
| Times Cited |
11
|
|
WOS Category
|
MICROBIOLOGY
|
| Resource |
| Pathogenic microorganisms |
IFM 0623
IFM 0624
IFM 0756
IFM 0757
IFM 0758
IFM 0759
IFM 0760
IFM 0761
IFM 0896
IFM 10098
IFM 10099
IFM 10700
IFM 11475
IFM 11477 |
