Reference - Detail
| RRC ID | 4024 |
|---|---|
| Author | Yu QB, Li G, Wang G, Sun JC, Wang PC, Wang C, Mi HL, Ma WM, Cui J, Cui YL, Chong K, Li YX, Li YH, Zhao Z, Shi TL, Yang ZN. |
| Title | Construction of a chloroplast protein interaction network and functional mining of photosynthetic proteins in Arabidopsis thaliana. |
| Journal | Cell Res |
| Abstract |
Chloroplast is a typical plant cell organelle where photosynthesis takes place. In this study, a total of 1,808 chloroplast core proteins in Arabidopsis thaliana were reliably identified by combining the results of previously published studies and our own predictions. We then constructed a chloroplast protein interaction network primarily based on these core protein interactions. The network had 22,925 protein interaction pairs which involved 2,214 proteins. A total of 160 previously uncharacterized proteins were annotated in this network. The subunits of the photosynthetic complexes were modularized, and the functional relationships among photosystem I (PSI), photosystem II (PSII), light harvesting complex of photosystem I (LHC I) and light harvesting complex of photosystem I (LHC II) could be deduced from the predicted protein interactions in this network. We further confirmed an interaction between an unknown protein AT1G52220 and a photosynthetic subunit PSI-D2 by yeast two-hybrid analysis. Our chloroplast protein interaction network should be useful for functional mining of photosynthetic proteins and investigation of chloroplast-related functions at the systems biology level in Arabidopsis. |
| Volume | 18(10) |
| Pages | 1007-19 |
| Published | 2008-10-1 |
| DOI | 10.1038/cr.2008.286 |
| PII | cr2008286 |
| PMID | 18813226 |
| MeSH | Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Chloroplasts / genetics* Chloroplasts / metabolism Green Fluorescent Proteins / analysis Green Fluorescent Proteins / biosynthesis Photosynthesis Photosynthetic Reaction Center Complex Proteins / genetics* Photosynthetic Reaction Center Complex Proteins / metabolism Photosystem I Protein Complex / genetics Photosystem I Protein Complex / metabolism Photosystem II Protein Complex / genetics Photosystem II Protein Complex / metabolism Two-Hybrid System Techniques |
| IF | 20.507 |
| Times Cited | 31 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda03205 pda02893 |