| RRC ID |
4036
|
| 著者 |
Xing DH, Lai ZB, Zheng ZY, Vinod KM, Fan BF, Chen ZX.
|
| タイトル |
Stress- and pathogen-induced Arabidopsis WRKY48 is a transcriptional activator that represses plant basal defense.
|
| ジャーナル |
Mol Plant
|
| Abstract |
Plant WRKY transcription factors can function as either positive or negative regulators of plant basal disease resistance. Arabidopsis WRKY48 is induced by mechanical and/or osmotic stress due to infiltration and pathogen infection and, therefore, may play a role in plant defense responses. WRKY48 is localized to the nucleus, recognizes the TTGACC W-box sequence with a high affinity in vitro and functions in plant cells as a strong transcriptional activator. To determine the biological functions directly, we have isolated loss-of-function T-DNA insertion mutants and generated gain-of-function transgenic overexpression plants for WRKY48 in Arabidopsis. Growth of a virulent strain of the bacterial pathogen Pseudomonas syringae was decreased in the wrky48 T-DNA insertion mutants. The enhanced resistance of the loss-of-function mutants was associated with increased induction of salicylic acid-regulated PR1 by the bacterial pathogen. By contrast, transgenic WRKY48-overexpressing plants support enhanced growth of P. syringae and the enhanced susceptibility was associated with reduced expression of defense-related PR genes. These results suggest that WRKY48 is a negative regulator of PR gene expression and basal resistance to the bacterial pathogen P. syringae.
|
| 巻・号 |
1(3)
|
| ページ |
459-70
|
| 公開日 |
2008-5-1
|
| DOI |
10.1093/mp/ssn020
|
| PII |
S1674-2052(14)60341-5
|
| PMID |
19825553
|
| MeSH |
Amino Acid Sequence
Arabidopsis / drug effects
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Base Sequence
DNA Probes
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Gene Expression Regulation, Plant*
Kinetics
Molecular Sequence Data
Molecular Weight
Plant Diseases / microbiology*
Plant Diseases / prevention & control
Pseudomonas syringae / pathogenicity*
RNA, Plant / chemistry
RNA, Plant / genetics
RNA, Ribosomal / chemistry
RNA, Ribosomal / genetics
Salicylic Acid / pharmacology
Sequence Alignment
Sequence Homology, Nucleic Acid
Trans-Activators / genetics
Trans-Activators / metabolism*
Transcription Factors / genetics
Transcription Factors / metabolism*
|
| IF |
12.084
|
| 引用数 |
80
|
|
WOS 分野
|
PLANT SCIENCES
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda03899 |
