RRC ID 4036
Author Xing DH, Lai ZB, Zheng ZY, Vinod KM, Fan BF, Chen ZX.
Title Stress- and pathogen-induced Arabidopsis WRKY48 is a transcriptional activator that represses plant basal defense.
Journal Mol Plant
Abstract Plant WRKY transcription factors can function as either positive or negative regulators of plant basal disease resistance. Arabidopsis WRKY48 is induced by mechanical and/or osmotic stress due to infiltration and pathogen infection and, therefore, may play a role in plant defense responses. WRKY48 is localized to the nucleus, recognizes the TTGACC W-box sequence with a high affinity in vitro and functions in plant cells as a strong transcriptional activator. To determine the biological functions directly, we have isolated loss-of-function T-DNA insertion mutants and generated gain-of-function transgenic overexpression plants for WRKY48 in Arabidopsis. Growth of a virulent strain of the bacterial pathogen Pseudomonas syringae was decreased in the wrky48 T-DNA insertion mutants. The enhanced resistance of the loss-of-function mutants was associated with increased induction of salicylic acid-regulated PR1 by the bacterial pathogen. By contrast, transgenic WRKY48-overexpressing plants support enhanced growth of P. syringae and the enhanced susceptibility was associated with reduced expression of defense-related PR genes. These results suggest that WRKY48 is a negative regulator of PR gene expression and basal resistance to the bacterial pathogen P. syringae.
Volume 1(3)
Pages 459-70
Published 2008-5
DOI 10.1093/mp/ssn020
PII S1674-2052(14)60341-5
PMID 19825553
MeSH Amino Acid Sequence Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Base Sequence DNA Probes DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Plant* Kinetics Molecular Sequence Data Molecular Weight Plant Diseases / microbiology* Plant Diseases / prevention & control Pseudomonas syringae / pathogenicity* RNA, Plant / chemistry RNA, Plant / genetics RNA, Ribosomal / chemistry RNA, Ribosomal / genetics Salicylic Acid / pharmacology Sequence Alignment Sequence Homology, Nucleic Acid Trans-Activators / genetics Trans-Activators / metabolism* Transcription Factors / genetics Transcription Factors / metabolism*
IF 9.326
Times Cited 41
Arabidopsis / Cultured plant cells, genes pda03899