| RRC ID |
4044
|
| 著者 |
Reumann S, Babujee L, Ma C, Wienkoop S, Siemsen T, Antonicelli GE, Rasche N, Lüder F, Weckwerth W, Jahn O.
|
| タイトル |
Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.
|
| ジャーナル |
Plant Cell
|
| Abstract |
We have established a protocol for the isolation of highly purified peroxisomes from mature Arabidopsis thaliana leaves and analyzed the proteome by complementary gel-based and gel-free approaches. Seventy-eight nonredundant proteins were identified, of which 42 novel proteins had previously not been associated with plant peroxisomes. Seventeen novel proteins carried predicted peroxisomal targeting signals (PTS) type 1 or type 2; 11 proteins contained PTS-related peptides. Peroxisome targeting was supported for many novel proteins by in silico analyses and confirmed for 11 representative full-length fusion proteins by fluorescence microscopy. The targeting function of predicted and unpredicted signals was investigated and SSL>, SSI>, and ASL> were established as novel functional PTS1 peptides. In contrast with the generally accepted confinement of PTS2 peptides to the N-terminal domain, the bifunctional transthyretin-like protein was demonstrated to carry internally a functional PTS2. The novel enzymes include numerous enoyl-CoA hydratases, short-chain dehydrogenases, and several enzymes involved in NADP and glutathione metabolism. Seven proteins, including beta-glucosidases and myrosinases, support the currently emerging evidence for an important role of leaf peroxisomes in defense against pathogens and herbivores. The data provide new insights into the biology of plant peroxisomes and improve the prediction accuracy of peroxisome-targeted proteins from genome sequences.
|
| 巻・号 |
19(10)
|
| ページ |
3170-93
|
| 公開日 |
2007-10-1
|
| DOI |
10.1105/tpc.107.050989
|
| PII |
tpc.107.050989
|
| PMID |
17951448
|
| PMC |
PMC2174697
|
| MeSH |
Amino Acid Sequence
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / metabolism*
Computational Biology
Electrophoresis, Gel, Two-Dimensional
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Peptides / chemistry
Peptides / metabolism
Peroxisome-Targeting Signal 1 Receptor
Peroxisomes / metabolism*
Plant Leaves / cytology
Plant Leaves / metabolism*
Proteome / analysis*
Proteomics / methods
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / metabolism
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
|
| IF |
9.618
|
| 引用数 |
229
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
PLANT SCIENCES
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda11037 |
