| RRC ID |
4052
|
| Author |
Tabuchi T, Okada T, Azuma T, Nanmori T, Yasuda T.
|
| Title |
Posttranscriptional regulation by the upstream open reading frame of the phosphoethanolamine N-methyltransferase gene.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
Phosphoethanolamine N-methyltransferase (PEAMT) is involved in choline biosynthesis in plants. The 5' untranslated region (UTR) of several PEAMT genes was found to contain an upstream open reading frame (uORF). We generated transgenic Arabidopsis calli that expressed a chimeric gene constructed by fusing the 5' UTR of the Arabidopsis PEAMT gene (AtNMT1) upstream of the beta-glucuronidase gene. The AtNMT1 uORF was found to be involved in declining levels of the chimeric gene mRNA and repression of downstream beta-glucuronidase gene translation in the calli when the cells were treated with choline. Further, we discuss the role of the uORF.
|
| Volume |
70(9)
|
| Pages |
2330-4
|
| Published |
2006-9-1
|
| DOI |
10.1271/bbb.60309
|
| PII |
JST.JSTAGE/bbb/60309
|
| PMID |
16960350
|
| MeSH |
5' Untranslated Regions / metabolism
Amino Acid Sequence
Arabidopsis / enzymology*
Arabidopsis / genetics
Arabidopsis / metabolism
Choline / metabolism
DNA, Plant / chemistry
DNA, Plant / genetics
Gene Expression Regulation, Plant / physiology*
Methyltransferases / genetics*
Methyltransferases / metabolism
Molecular Sequence Data
Mutant Chimeric Proteins / genetics
Mutant Chimeric Proteins / metabolism
Nucleic Acid Hybridization
Open Reading Frames*
Point Mutation
Transcription, Genetic
|
| IF |
1.516
|
| Times Cited |
32
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
