| RRC ID |
4053
|
| 著者 |
Martin T, Sharma R, Sippel C, Waegemann K, Soll J, Vothknecht UC.
|
| タイトル |
A protein kinase family in Arabidopsis phosphorylates chloroplast precursor proteins.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
A serine/threonine protein kinase that is able to phosphorylate chloroplast-destined precursor proteins was purified from leaf extract of Arabidopsis thaliana and was identified by mass spectrometry. The protein kinase, encoded by AT2G17700, belongs to a small protein family comprising in addition AT4G35780 and AT4G38470. All three proteins were expressed heterologously in Escherichia coli and characterized with regard to their properties in precursor protein phosphorylation. They were able to phosphorylate several chloroplast-destined precursor proteins within their cleavable presequences. In contrast, a mitochondria-destined precursor protein was not a substrate for these kinases. For all three enzymes, the phosphorylation reaction was specific for ATP with apparent K(m) values between 14 and 67 microM. They did not utilize other NTPs nor were those able to compete for ATP in the reaction. An excess of ADP was able to inhibit ATP-dependent phosphorylation. Furthermore, all three kinases exhibited autophosphorylation. The protein kinases described here could represent subunits of a regulatory network involved in the cytosolic events of chloroplast protein import.
|
| 巻・号 |
281(52)
|
| ページ |
40216-23
|
| 公開日 |
2006-12-29
|
| DOI |
10.1074/jbc.M606580200
|
| PII |
S0021-9258(20)76858-4
|
| PMID |
17090544
|
| MeSH |
Amino Acid Sequence
Arabidopsis Proteins / metabolism*
Chloroplasts / enzymology*
Chloroplasts / metabolism
Cytosol / enzymology
Isoenzymes / metabolism
Molecular Sequence Data
Multigene Family
Phosphorylation
Plant Leaves / enzymology
Plant Leaves / metabolism
Protein Precursors / metabolism*
Protein Serine-Threonine Kinases / metabolism*
Protein Subunits / metabolism
Protein Transport
|
| IF |
4.238
|
| 引用数 |
50
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda09214 |
