論文 - 詳細
| RRC ID | 4055 |
|---|---|
| 著者 | Imai H, Nishiura H. |
| タイトル | Phosphorylation of sphingoid long-chain bases in Arabidopsis: functional characterization and expression of the first sphingoid long-chain base Kinase gene in plants. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
Sphingoid long-chain base (LCB) kinase catalyzes the phosphorylation of LCBs to form LCB 1-phosphates. Based on sequence identity to murine sphingosine kinase (mSPHK1), we cloned and characterized the first plant LCB kinase gene from Arabidopsis (AtLCBK1). Using recombinant AtLCBK1 protein from Escherichia coli cells, we confirmed that the enzyme specifically phosphorylated D-erythro-dihydrosphingosine (DHS), but not N-acetyl-DHS or D-threo-DHS. AtLCBK1 also phosphorylated D-erythro-sphingosine, trans-4, trans-8-sphingadienine and phytosphingosine. We found that AtLCBK1 mRNA is highly expressed in flowers. AtLCBK1 transcripts were slightly increased by low humidity or abscisic acid treatments, suggesting that AtLCBK1 is constitutively expressed under these treatments. |
| 巻・号 | 46(2) |
| ページ | 375-80 |
| 公開日 | 2005-2-1 |
| DOI | 10.1093/pcp/pci023 |
| PII | pci023 |
| PMID | 15695468 |
| MeSH | Amino Acid Sequence Arabidopsis / enzymology* Arabidopsis / genetics* Conserved Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Phosphorylation Phosphotransferases (Alcohol Group Acceptor) / chemistry Phosphotransferases (Alcohol Group Acceptor) / genetics* Phosphotransferases (Alcohol Group Acceptor) / metabolism* |
| IF | 4.062 |
| 引用数 | 32 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00008 |